Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613151643_ay1w CACACACACACACACACACCCCAGTAGTCAGG(+AGGACA)GTACCCTTTTAATTTTTGTTTGAAATCAACT NGG 10 8 17 8 24 0 100 5 10
    240613151641_475z GGCCAGATCGACCAGAAATGGCATCTGCCATG(+AGGACA)GTACCTGCTGTTTCTAGTAGCAGGCCATGAT NGG 10 8 17 8 24 0 100 5 10
    240613151640_nmqv AAGACATCATGGCCTGCTACTAGAAACAGCAG(+AGGACA)GTACCATGGCAGATGCCATTTCTGGTCGATC NGG 10 8 17 8 24 0 100 5 10
    240613151639_3s3x GCTCGTACATTGGTCCTTGCCTTCTGGAGGAG(+AGGACA)GTACCCAGGGCGGTCCTGCTTCCTCTCTTCA NGG 10 8 17 8 24 0 100 5 10
    240613151635_mr45 GATGATGAAGAGAGGAAGCAGGACCGCCCTGG(+AGGACA)GTACCTCCTCCAGAAGGCAAGGACCAATGTA NGG 10 8 17 8 24 0 100 5 10
    240613151634_hpx5 AATTTCAGGATGTCTTTATGAGAACCTGATTG(+AGGACA)GATCCTACGTACAAGTAGGATGGTGGTGGCG NGG 10 8 17 8 24 0 100 5 10
    240613151633_onbr CTGACCGCCACCACCATCCTACTTGTACGTAG(+AGGACA)GATCCAATCAGGTTCTCATAAAGACATCCTG NGG 10 8 17 8 24 0 100 5 10
    240613151632_diw6 ACGGACAGTGGAAGACCCAGGGCTGAGGAGAG(+AGGACA)GATCCCTTCCAAGGACTCTGAGAAACTAGAC NGG 10 8 17 8 24 0 100 5 10
    240613151630_0bs6 GAAGGGTCTAGTTTCTCAGAGTCCTTGGAAGG(+AGGACA)GATCCTCTCCTCAGCCCTGGGTCTTCCACTG NGG 10 8 17 8 24 0 100 5 10
    240613151629_jjzw TCATATAGAATCTAGGCGTGGCCTCCCTCCTG(+AGGACA)GTACCATTCTGGTATCCAACACCAATTACTT NGG 10 8 17 8 24 0 100 5 10