Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613151554_1ja9 CACCACTCATCACCTAGCACCCTGGCTATATG(+AGGACA)GTACCTCAACAAATAAATTAAAGATTTCCAT NGG 10 8 17 8 24 0 100 5 10
    240613151552_bgvt AACTACTGGTTTGCTGTGTAAATGGATGTCAG(+AGGACA)GATCCAGCAAAACTACTGCTTTCTTCTCCAA NGG 10 8 17 8 24 0 100 5 10
    240613151551_5sjg TGTCTTTGGAGAAGAAAGCAGTAGTTTTGCTG(+AGGACA)GATCCTGACATCCATTTACACAGCAAACCAG NGG 10 8 17 8 24 0 100 5 10
    240613151550_8w80 CCTACTAGGTTCTAACTGATGCAGAGTGAAGG(+AGGACA)GTACCGAGAAAGTACATGTACATAAAGATTT NGG 10 8 17 8 24 0 100 5 10
    240613151548_uigg CTCTTAAATCTTTATGTACATGTACTTTCTCG(+AGGACA)GTACCCTTCACTCTGCATCAGTTAGAACCTA NGG 10 8 17 8 24 0 100 5 10
    240613151547_kpv9 GGCTGAGTGGAAGTTTGGTTTTAGGAAGTAAG(+AGGACA)GTACCATACGACATTAATCTTTCAGTGAGTA NGG 10 8 17 8 24 0 100 5 10
    240613151545_jcls GGGTTTACTCACTGAAAGATTAATGTCGTATG(+AGGACA)GTACCTTACTTCCTAAAACCAAACTTCCACT NGG 10 8 17 8 24 0 100 5 10
    240613151544_jf2o GGCAGAGAGACTGTCCTCCATTTTAAGACTTG(+AGGACA)GTACCACTTTGAGAGATGAGTCCAGGGTCCC NGG 10 8 17 8 24 0 100 5 10
    240613151543_ljx9 GCTGTGGGACCCTGGACTCATCTCTCAAAGTG(+AGGACA)GTACCAAGTCTTAAAATGGAGGACAGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240613151542_pf1u AATTTTCATTTGTGTAAACTCCAAAAAAAAAG(+AGGACA)GATCCTAACAACTATTTTGGGTTTTATCATT NGG 10 8 17 8 24 0 100 5 10