Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613151753_r96s TGATATTTTCAGCCATAGCCAGCTCCTTCTGG(+AGGACA)GTACCTTATGACCCCTGGCTCCACTCCAGAT NGG 10 8 17 8 24 0 100 5 10
    240613151752_98e8 CTCTGGGGTCAAATCAATTCCTCTCAAGTGTG(+AGGACA)GTACCCTAGAGAGTGGTGGCCATCTACATTT NGG 10 8 17 8 24 0 100 5 10
    240613151750_qrn1 GGAGAAAATGTAGATGGCCACCACTCTCTAGG(+AGGACA)GTACCACACTTGAGAGGAATTGATTTGACCC NGG 10 8 17 8 24 0 100 5 10
    240613151748_n810 GGCTCCGATTCCTTCACATTCCAGCTGGCCCG(+AGGACA)GATCCCTGTGTGGCCATAAGGCCTTGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240613151746_dciz GTGACGAGGGCAAGGCCTTATGGCCACACAGG(+AGGACA)GATCCGGGCCAGCTGGAATGTGAAGGAATCG NGG 10 8 17 8 24 0 100 5 10
    240613151744_u8q5 GGGGCACATCTGTGGAGAGGCAGAAGCCAAGG(+AGGACA)GATCCTGGCTGTGCCCTATGATTCCCAGATC NGG 10 8 17 8 24 0 100 5 10
    240613151742_dz86 GTCAAGATCTGGGAATCATAGGGCACAGCCAG(+AGGACA)GATCCCTTGGCTTCTGCCTCTCCACAGATGT NGG 10 8 17 8 24 0 100 5 10
    240613151740_pv17 TTTTTACAAGGCTTATAAATCCTTTAGTGTGG(+AGGACA)GATCCTGTCCCACCATCGGCAGCTGTGGTTG NGG 10 8 17 8 24 0 100 5 10
    240613151739_0r8l TGTCCCAACCACAGCTGCCGATGGTGGGACAG(+AGGACA)GATCCCACACTAAAGGATTTATAAGCCTTGT NGG 10 8 17 8 24 0 100 5 10
    240613151738_9h2u ACCAATGGCACCAGGGTCAGAAGACTGACTTG(+AGGACA)GATCCGTCACAGAACCCTTTTCAGAGGCTGC NGG 10 8 17 8 24 0 100 5 10