Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613151453_3jbv AAATATCTTAACTATAAACATCATCTTTTATG(+AGGACA)GATCCTGAGACCAGGTTTCCATGACCTCATA NGG 10 8 17 8 24 0 100 5 10
    240613151451_1db8 TGGTCTTTATTTAGAGTCCATAACCCCTCCAG(+AGGACA)GTACCTCAATCCTTCCCATAAATCTTCCACA NGG 10 8 17 8 24 0 100 5 10
    240613151450_6i4s GATCTTGTGGAAGATTTATGGGAAGGATTGAG(+AGGACA)GTACCTGGAGGGGTTATGGACTCTAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240613151449_fp8h GGTTAGACAGGCCAGCCCAGCTAGGAGAGCAG(+AGGACA)GATCCACAGGTAGACGGCAGAGTCATGGTAA NGG 10 8 17 8 24 0 100 5 10
    240613151447_8e2d GTGGCTTACCATGACTCTGCCGTCTACCTGTG(+AGGACA)GATCCTGCTCTCCTAGCTGGGCTGGCCTGTC NGG 10 8 17 8 24 0 100 5 10
    240613151446_wibo GAAACAGCCAAAGAATGGATAAAGAAAATGAG(+AGGACA)GTACCTTTACATAATGGAATATTACTCAAAT NGG 10 8 17 8 24 0 100 5 10
    240613151445_5iir TTAATATTTGAGTAATATTCCATTATGTAAAG(+AGGACA)GTACCTCATTTTCTTTATCCATTCTTTGGCT NGG 10 8 17 8 24 0 100 5 10
    240613151443_nqe2 ATAATAGGGTAATGCTTAGTAGAAGGCACGTG(+AGGACA)GATCCATACACTAGACTAACTCGGGAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613151442_3rrv AATTTTCCACTCCCGAGTTAGTCTAGTGTATG(+AGGACA)GATCCACGTGCCTTCTACTAAGCATTACCCT NGG 10 8 17 8 24 0 100 5 10
    240613151441_r4ee ATTTTATTGCAGTGATACAGATGCCACTGTTG(+AGGACA)GTACCCTTAAATTTTTATTTCTGCTCACCAA NGG 10 8 17 8 24 0 100 5 10