Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613151439_ykq3 TAACCTTGGTGAGCAGAAATAAAAATTTAAGG(+AGGACA)GTACCAACAGTGGCATCTGTATCACTGCAAT NGG 10 8 17 8 24 0 100 5 10
    240613151437_6xkk TGAGACTCTGTGAAGGGCTGAGCAAAGGGAGG(+AGGACA)GTACCAAGCCCTGGAGAACCTGCCCTGAAAG NGG 10 8 17 8 24 0 100 5 10
    240613151436_qvu9 CCTGGCTTTCAGGGCAGGTTCTCCAGGGCTTG(+AGGACA)GTACCCTCCCTTTGCTCAGCCCTTCACAGAG NGG 10 8 17 8 24 0 100 5 10
    240613151435_m5kv GTGATTTAGCTAAAATCTACTGGAATCATATG(+AGGACA)GTACCTGTGATTTCGGAGGTAGGGTACAACA NGG 10 8 17 8 24 0 100 5 10
    240613151433_4245 CATTATGTTGTACCCTACCTCCGAAATCACAG(+AGGACA)GTACCATATGATTCCAGTAGATTTTAGCTAA NGG 10 8 17 8 24 0 100 5 10
    240613151431_z6qj CGTACAAAGTGGGCGGTGCTGAGGGGCTTGGG(+AGGACA)GATCCTTGCTGCAAAGCTTTGCAGAATGTAA NGG 10 8 17 8 24 0 100 5 10
    240613151430_c4d5 GCCTCTTACATTCTGCAAAGCTTTGCAGCAAG(+AGGACA)GATCCCCAAGCCCCTCAGCACCGCCCACTTT NGG 10 8 17 8 24 0 100 5 10
    240613151429_wcgq CCCTTCAGACTGAGTAAACTTTTCAATATATG(+AGGACA)GTACCCAGATGTTTTATTTATGAAGTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240613151427_cd11 TAAAATAAAAACTTCATAAATAAAACATCTGG(+AGGACA)GTACCATATATTGAAAAGTTTACTCAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240613151425_s691 AAACAAACCAAACAAAACATAAAAAGCCTTTG(+AGGACA)GTACCTTTCAATAACAAAAGAAAAATATATT NGG 10 8 17 8 24 0 100 5 10