Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613151411_u3ez AGAGAAGCTAGGCTTCTGCCGGGGCCTCTGAG(+AGGACA)GATCCATTGCCCTTGCCCAGAAGAGAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240613151410_fgxt GTCTTCACACTCTCCTGTATGTATCTGTATTG(+AGGACA)GTACCAATGAAATACAGTTCAGCATTTAATA NGG 10 8 17 8 24 0 100 5 10
    240613151409_h7uv TAGCATATTAAATGCTGAACTGTATTTCATTG(+AGGACA)GTACCAATACAGATACATACAGGAGAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240613151407_jgun TCTCCCAACTATTCATAATTCTAGTTCAAGTG(+AGGACA)GATCCAGCACCCTCTTCTAACTGATACTGCC NGG 10 8 17 8 24 0 100 5 10
    240613151406_mfdx GTACTGGCAGTATCAGTTAGAAGAGGGTGCTG(+AGGACA)GATCCACTTGAACTAGAATTATGAATAGTTG NGG 10 8 17 8 24 0 100 5 10
    240613151403_2vv3 TTTGTAGACCAGGCTGGCCTTGAAGTCCCAGG(+AGGACA)GATCCTGTCTGTGCCTCTCACATGTGTTAAG NGG 10 8 17 8 24 0 100 5 10
    240613151402_6r4k TTAGTCTTAACACATGTGAGAGGCACAGACAG(+AGGACA)GATCCCTGGGACTTCAAGGCCAGCCTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240613151400_5hfs TCCTGGCAGGTGATCTACAAAGCAGAAGCGTG(+AGGACA)GTACCCAGTCAGTCACAGAGAATAGCATCCT NGG 10 8 17 8 24 0 100 5 10
    240613151359_rk44 AATCAAGGATGCTATTCTCTGTGACTGACTGG(+AGGACA)GTACCACGCTTCTGCTTTGTAGATCACCTGC NGG 10 8 17 8 24 0 100 5 10
    240613151357_kku4 CACACTGTAAGAGGTCCCTAGTCTGTGTGGTG(+AGGACA)GTACCTTTAATGCCAGCACTTGGGAGGCAGA NGG 10 8 17 8 24 0 100 5 10