Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613151325_4skr GGTTCTTGAGCTGCCAGGGTTCCAGGGCAGGG(+AGGACA)GATCCTAAAGCTCAGAGCAATGAGGAATGCA NGG 10 8 17 8 24 0 100 5 10
    240613151324_75yh GGGTGAGGGGAGGTCCCTGCCACAAAACAAAG(+AGGACA)GTACCAGGAACAAAACCGAGCCTTCAGGAAT NGG 10 8 17 8 24 0 100 5 10
    240613151322_g2mj TTGCAATTCCTGAAGGCTCGGTTTTGTTCCTG(+AGGACA)GTACCTTTGTTTTGTGGCAGGGACCTCCCCT NGG 10 8 17 8 24 0 100 5 10
    240613151320_6530 GTTCTGTCATTTGCTTTCTAAAGCAGTGCCAG(+AGGACA)GTACCATGGAATGTTCACGGCTCCAAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613151318_u5jt GGTGGTTCCTTTGGAGCCGTGAACATTCCATG(+AGGACA)GTACCTGGCACTGCTTTAGAAAGCAAATGAC NGG 10 8 17 8 24 0 100 5 10
    240613151317_p1fb ACAAAGGATGAGCCCTGCTGTGTTGCAAGTAG(+AGGACA)GATCCCCTCCCCCAGGTCTATGAGGCTAATA NGG 10 8 17 8 24 0 100 5 10
    240613151315_lb68 AATCTTATTAGCCTCATAGACCTGGGGGAGGG(+AGGACA)GATCCTACTTGCAACACAGCAGGGCTCATCC NGG 10 8 17 8 24 0 100 5 10
    240613151314_vc2y AACAGATGAAATCTTTCATGGCTGAAATCTTG(+AGGACA)GATCCCTAAATGACCCGCCACCGTGGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240613151312_m7ry TTATAAAAGCCCACGGTGGCGGGTCATTTAGG(+AGGACA)GATCCAAGATTTCAGCCATGAAAGATTTCAT NGG 10 8 17 8 24 0 100 5 10
    240613151311_yc9r AGAGCATCCCTCTGGAGGTAAGGTCCTCTCAG(+AGGACA)GTACCTTCTCACACCATCCCCAGTTACACCA NGG 10 8 17 8 24 0 100 5 10