Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613151223_s6vg CTGCTCAAAGGGGGTTCTTGGAACTGAACTTG(+AGGACA)GATCCTCTGAAAGAGCAGTATGTGTGCTTAG NGG 10 8 17 8 24 0 100 5 10
    240613151221_u1y2 GCCCTGGTTGAGTGTCGTGTGTCACACCTGTG(+AGGACA)GTACCTGCTCAGATTTATTTCACTTCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240613151220_hr0e GTAAACCAAAGAAGTGAAATAAATCTGAGCAG(+AGGACA)GTACCACAGGTGTGACACACGACACTCAACC NGG 10 8 17 8 24 0 100 5 10
    240613151219_izur AGCCGAACCACAGATGGCTTCGAGTAAGTCTG(+AGGACA)GATCCTGACACCCGCCCTCCCCACCCCGAGC NGG 10 8 17 8 24 0 100 5 10
    240613151217_6nff CTGAAGCTCGGGGTGGGGAGGGCGGGTGTCAG(+AGGACA)GATCCAGACTTACTCGAAGCCATCTGTGGTT NGG 10 8 17 8 24 0 100 5 10
    240613151216_df4g CTGGTAAACTTGCCTTCCCGTTTCTCTGCCTG(+AGGACA)GTACCTTATTTGAGCAGGCTGTCAGCAATGG NGG 10 8 17 8 24 0 100 5 10
    240613151214_g58b AAAGGCCATTGCTGACAGCCTGCTCAAATAAG(+AGGACA)GTACCAGGCAGAGAAACGGGAAGGCAAGTTT NGG 10 8 17 8 24 0 100 5 10
    240613151213_pl5r CTTGCAAAGTGCTCAGGAACACAGAATGTCTG(+AGGACA)GATCCTGATGTCAAGCAGGGTCCGGGAGCTT NGG 10 8 17 8 24 0 100 5 10
    240613151212_g17i TGTGCAAGCTCCCGGACCCTGCTTGACATCAG(+AGGACA)GATCCAGACATTCTGTGTTCCTGAGCACTTT NGG 10 8 17 8 24 0 100 5 10
    240613151211_8dhc TTCCTACTTGAAGCCCTTGCCCGTTCCAGCTG(+AGGACA)GATCCTTGGTGAGGACTACCGAGGTTTAACC NGG 10 8 17 8 24 0 100 5 10