Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613151152_ofdy AGAGAAGGTGCATGAAAGGATCATTTCACAGG(+AGGACA)GTACCCTCTTACCCTTCTATGTTTCTTTGAC NGG 10 8 17 8 24 0 100 5 10
    240613151151_d9a3 AGTGACAGATTAACAGGGGTAGCTGCTACTTG(+AGGACA)GATCCACAGAATCTCTGCAAGTGAGGGTTAA NGG 10 8 17 8 24 0 100 5 10
    240613151150_1y56 CATAGTTAACCCTCACTTGCAGAGATTCTGTG(+AGGACA)GATCCAAGTAGCAGCTACCCCTGTTAATCTG NGG 10 8 17 8 24 0 100 5 10
    240613151149_8qjo CAGCCCCAGCAAGCGCCCAGGAACAGGGAGAG(+AGGACA)GATCCATGCATGTGAGCAGCCAGCATCAAGG NGG 10 8 17 8 24 0 100 5 10
    240613151148_0bxq GGTGTCCTTGATGCTGGCTGCTCACATGCATG(+AGGACA)GATCCTCTCCCTGTTCCTGGGCGCTTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240613151147_5ekk TTGATATAAAGCACAAACTGGCTTTAAACTCG(+AGGACA)GATCCTTCGGCCTCAGCCTTTCTAGCACCAA NGG 10 8 17 8 24 0 100 5 10
    240613151145_n764 TCTCCTTGGTGCTAGAAAGGCTGAGGCCGAAG(+AGGACA)GATCCGAGTTTAAAGCCAGTTTGTGCTTTAT NGG 10 8 17 8 24 0 100 5 10
    240613151143_idy8 TGTGGGTAGAAAATGGTAACACTAAACAATGG(+AGGACA)GATCCCAGTACTCAACTTTAAAGATTTTATT NGG 10 8 17 8 24 0 100 5 10
    240613151141_2aqb GAGAAAATAAAATCTTTAAAGTTGAGTACTGG(+AGGACA)GATCCCATTGTTTAGTGTTACCATTTTCTAC NGG 10 8 17 8 24 0 100 5 10
    240613151139_ohu3 ACACCCCTGAAACTGACAAGTGAGGGGCCAGG(+AGGACA)GATCCCCCACTGACTGGATTGTGTCTGGGCA NGG 10 8 17 8 24 0 100 5 10