Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613151108_n5vg CCTGAACACGACACTTCTCTTTTAAGGAGGAG(+AGGACA)GTACCAATGGTCAATTTCAAAGCATCAAGTC NGG 10 8 17 8 24 0 100 5 10
    240613151106_exil CTGGGTGTGCAGGAATTCTGCCTGTGAAGTGG(+AGGACA)GATCCACAGCATGTTCGACGGGTGGGAGAAC NGG 10 8 17 8 24 0 100 5 10
    240613151105_cr7a GCCCAGTTCTCCCACCCGTCGAACATGCTGTG(+AGGACA)GATCCCACTTCACAGGCAGAATTCCTGCACA NGG 10 8 17 8 24 0 100 5 10
    240613151104_012s CTTGTTCTACCATCTCTCCTGGGTTCTTCATG(+AGGACA)GTACCACCTCGTGTACAGCCACGCCAGTTAA NGG 10 8 17 8 24 0 100 5 10
    240613151102_ss1m CTGGTTTAACTGGCGTGGCTGTACACGAGGTG(+AGGACA)GTACCATGAAGAACCCAGGAGAGATGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240613151100_xy7x CTCCGTACACTGTGTGGGCAGTGTGGCCTTGG(+AGGACA)GATCCCCACTGATTACCAGGGATGGATGCCT NGG 10 8 17 8 24 0 100 5 10
    240613151059_tvkt GGGACAGGCATCCATCCCTGGTAATCAGTGGG(+AGGACA)GATCCCAAGGCCACACTGCCCACACAGTGTA NGG 10 8 17 8 24 0 100 5 10
    240613151057_3603 GTCCTTTATCCTCTGAATCGTTGTTCTGTTGG(+AGGACA)GATCCGTGCTGTGCTGTTCTGTGGAAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240613151056_abss TGGGGCCACTTTCCACAGAACAGCACAGCACG(+AGGACA)GATCCCAACAGAACAACGATTCAGAGGATAA NGG 10 8 17 8 24 0 100 5 10
    240613151055_zgor TAGCCTCCCGCACTAGGAATGCCCTTTATTTG(+AGGACA)GATCCAGATGATAAGTTGATAGTCATTAAAG NGG 10 8 17 8 24 0 100 5 10