Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613151123_g7dr GTCATGACCAGAAACAAGACGCGCCTGCTTGG(+AGGACA)GTACCACTTACAGGACCTGAAACATGCTTAG NGG 10 8 17 8 24 0 100 5 10
    240613151121_99b8 TAAAGTTAACAGTACAGCACAGCCCACATGTG(+AGGACA)GATCCATACCAAATCTATCTGATAAATAGTT NGG 10 8 17 8 24 0 100 5 10
    240613151120_8eci GTTTCAACTATTTATCAGATAGATTTGGTATG(+AGGACA)GATCCACATGTGGGCTGTGCTGTACTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240613151119_qifr GGAGAGCCCTGCTGCTTTCTGATGTTTGGAAG(+AGGACA)GTACCCAGCATCCTGATTCCCTCCAGTTCAT NGG 10 8 17 8 24 0 100 5 10
    240613151117_s9xd GCCAAATGAACTGGAGGGAATCAGGATGCTGG(+AGGACA)GTACCTTCCAAACATCAGAAAGCAGCAGGGC NGG 10 8 17 8 24 0 100 5 10
    240613151115_jzmr GTCGGGCCCCCAACAACTTGAAGCTTCCTCTG(+AGGACA)GATCCTCACTTGAGCTCTTTGGGGATGGGGC NGG 10 8 17 8 24 0 100 5 10
    240613151114_ykiy CCTAGGCCCCATCCCCAAAGAGCTCAAGTGAG(+AGGACA)GATCCAGAGGAAGCTTCAAGTTGTTGGGGGC NGG 10 8 17 8 24 0 100 5 10
    240613151113_2c9k CTATGCTACCCCACTTCCTGTATGCTCAACTG(+AGGACA)GATCCTCAAGACCATTTCATCTCTAGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240613151111_a511 CGTGGTGCAGCTAGAGATGAAATGGTCTTGAG(+AGGACA)GATCCAGTTGAGCATACAGGAAGTGGGGTAG NGG 10 8 17 8 24 0 100 5 10
    240613151110_t5lf TGACAGACTTGATGCTTTGAAATTGACCATTG(+AGGACA)GTACCTCCTCCTTAAAAGAGAAGTGTCGTGT NGG 10 8 17 8 24 0 100 5 10