Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613151001_b4eh GCTCCAATGCCCACAGGTTTTCTCAAGAGAAG(+AGGACA)GATCCTCAAGTTAAAAGTTCAGCAAGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240613151000_ugq4 CAGAGTATATATTATGTACACACATACATATG(+AGGACA)GATCCATACACGTAAGTGCACATACGTACAC NGG 10 8 17 8 24 0 100 5 10
    240613150959_tem8 TGTGTGTGTACGTATGTGCACTTACGTGTATG(+AGGACA)GATCCATATGTATGTGTGTACATAATATATA NGG 10 8 17 8 24 0 100 5 10
    240613150956_sijw AAGGACCATTGCATGTCATAAGACCTGCCCTG(+AGGACA)GATCCCTTTAAAAGTCTTTTCACAGGACACT NGG 10 8 17 8 24 0 100 5 10
    240613150955_ntsw GAATTAGTGTCCTGTGAAAAGACTTTTAAAGG(+AGGACA)GATCCAGGGCAGGTCTTATGACATGCAATGG NGG 10 8 17 8 24 0 100 5 10
    240613150953_bifi TCAATGAGAAATATGGTCCTGCTCTAGTCCTG(+AGGACA)GATCCAAGAGAGGCAAGAAACCAGCGGCCGC NGG 10 8 17 8 24 0 100 5 10
    240613150952_vmmz GATGAGCGGCCGCTGGTTTCTTGCCTCTCTTG(+AGGACA)GATCCAGGACTAGAGCAGGACCATATTTCTC NGG 10 8 17 8 24 0 100 5 10
    240613150950_gdbs TTGCCTGCATGTCTGTCTGTGTGAGGGTGGAG(+AGGACA)GATCCCCTGGAGTCATAGAAAGTTGTGAGGT NGG 10 8 17 8 24 0 100 5 10
    240613150947_3i5v ATGGCACCTCACAACTTTCTATGACTCCAGGG(+AGGACA)GATCCTCCACCCTCACACAGACAGACATGCA NGG 10 8 17 8 24 0 100 5 10
    240613150945_3116 TATTTGTAGAGGGTAAAACCTGAGCCTGCCGG(+AGGACA)GATCCTCCCCCCAACCCCCTGCTTGCCCGGG NGG 10 8 17 8 24 0 100 5 10