Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613150914_ho6m TTTCACTTTTGCCCCCAAACAGTTTACATGTG(+AGGACA)GATCCACCGTAAGCAACTGGGTTTAATGTAG NGG 10 8 17 8 24 0 100 5 10
    240613150913_z0lg CACTCACACTGGGAGGCATTGTTAAGTGAACG(+AGGACA)GTACCGCAGGCTCAGATTTGAGCACTTCTGT NGG 10 8 17 8 24 0 100 5 10
    240613150911_jx74 GGTAGACAGAAGTGCTCAAATCTGAGCCTGCG(+AGGACA)GTACCGTTCACTTAACAATGCCTCCCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240613150910_xbp6 AAATCCCAGCACTTGGGAGGAAGAGGCAGGAG(+AGGACA)GATCCGAGTCTACATAGCCAGCTTGGTGTAC NGG 10 8 17 8 24 0 100 5 10
    240613150908_5j78 ATTATGTACACCAAGCTGGCTATGTAGACTCG(+AGGACA)GATCCTCCTGCCTCTTCCTCCCAAGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240613150907_8r0a AATTGATTTTTACTCTTTACTATCCAAGTTTG(+AGGACA)GATCCATTCAGAAGAGCTGGTTTTAAATTCT NGG 10 8 17 8 24 0 100 5 10
    240613150906_wvco CGAATAGAATTTAAAACCAGCTCTTCTGAATG(+AGGACA)GATCCAAACTTGGATAGTAAAGAGTAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240613150905_cddn AAGTGCACCTTTGTTATATACTTATTATATTG(+AGGACA)GTACCAAATACAGAAGAAAACTATAGTTCTG NGG 10 8 17 8 24 0 100 5 10
    240613150904_6ogi TATCACAGAACTATAGTTTTCTTCTGTATTTG(+AGGACA)GTACCAATATAATAAGTATATAACAAAGGTG NGG 10 8 17 8 24 0 100 5 10
    240613150902_tz5f GCAAGCAATTGCAGCACCTTCAGAATGTGGAG(+AGGACA)GTACCGGGCTAGACCTAGCAAACAATGTCCT NGG 10 8 17 8 24 0 100 5 10