Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613150813_gf6l TTACCACCGAGGTCACTTCACTGAGGTAACTG(+AGGACA)GTACCTTAGTCTCTTGTGGCCACTTACAGAT NGG 10 8 17 8 24 0 100 5 10
    240613150812_omo4 CCGATATCTGTAAGTGGCCACAAGAGACTAAG(+AGGACA)GTACCAGTTACCTCAGTGAAGTGACCTCGGT NGG 10 8 17 8 24 0 100 5 10
    240613150811_m0fy GCATTCCTCTTGCCCAGCCATGTTCACGGTTG(+AGGACA)GATCCTTAATGCATTGTGAGTGTTCCATCAA NGG 10 8 17 8 24 0 100 5 10
    240613150809_hvku AAAGTTTGATGGAACACTCACAATGCATTAAG(+AGGACA)GATCCAACCGTGAACATGGCTGGGCAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240613150808_g8wi TGAGGTTCATTGCTTAAGTCAATGTGGTAGTG(+AGGACA)GTACCCACTTATAATTCCAGTGTTTGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613150807_2kvr CTCAGCCTCCCAAACACTGGAATTATAAGTGG(+AGGACA)GTACCACTACCACATTGACTTAAGCAATGAA NGG 10 8 17 8 24 0 100 5 10
    240613150805_493b TCTCCTCCCCCTCGACCCTCCTAAATAACACG(+AGGACA)GTACCAGGCTTGCTGGCTGTGGGATTTACAG NGG 10 8 17 8 24 0 100 5 10
    240613150804_embh TATACCTGTAAATCCCACAGCCAGCAAGCCTG(+AGGACA)GTACCGTGTTATTTAGGAGGGTCGAGGGGGA NGG 10 8 17 8 24 0 100 5 10
    240613150801_wvek TTGGTGGGAAGTCTGCTGGAAGAGGCTTCCGG(+AGGACA)GTACCATTTAGGGGAAGTCAAACTTGTGACT NGG 10 8 17 8 24 0 100 5 10
    240613150800_m3xf TTTATAGTCACAAGTTTGACTTCCCCTAAATG(+AGGACA)GTACCCGGAAGCCTCTTCCAGCAGACTTCCC NGG 10 8 17 8 24 0 100 5 10