Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613150929_tjpz CAATCCTCTTTGGTTGGTAATAGACTTTTTTG(+AGGACA)GATCCATGTTTGATTTGAATCTAGCTACTTA NGG 10 8 17 8 24 0 100 5 10
    240613150928_sj31 ATGCTGTGCCTCTCATCCAAGTATTAACAGAG(+AGGACA)GTACCTGCCATCTTGGGAACCTTAAAGGAAT NGG 10 8 17 8 24 0 100 5 10
    240613150926_ekc2 TAGTTATTCCTTTAAGGTTCCCAAGATGGCAG(+AGGACA)GTACCTCTGTTAATACTTGGATGAGAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240613150925_0xgh AACACTTTTAAAAAAATTCATTTATAAATCAG(+AGGACA)GTACCCATTCCCACAATCTTAGGGTCTAGTC NGG 10 8 17 8 24 0 100 5 10
    240613150923_or0t ATCCAGACTAGACCCTAAGATTGTGGGAATGG(+AGGACA)GTACCTGATTTATAAATGAATTTTTTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240613150922_8h8d CAGGAGAGGTTCCTGGTCTGTGTCAAAGTCTG(+AGGACA)GTACCATAAAAACCAAGTAAGGATGGATCTG NGG 10 8 17 8 24 0 100 5 10
    240613150921_xr7g CCTGTCAGATCCATCCTTACTTGGTTTTTATG(+AGGACA)GTACCAGACTTTGACACAGACCAGGAACCTC NGG 10 8 17 8 24 0 100 5 10
    240613150919_dy6c CTACAAAGTCTCTCCTGTGCCTTCCCAGATAG(+AGGACA)GTACCTAGGCTGCATCCTGGAGCCACGTTCC NGG 10 8 17 8 24 0 100 5 10
    240613150917_r7o4 GCTATGGAACGTGGCTCCAGGATGCAGCCTAG(+AGGACA)GTACCTATCTGGGAAGGCACAGGAGAGACTT NGG 10 8 17 8 24 0 100 5 10
    240613150916_ghhg TCTTTCTACATTAAACCCAGTTGCTTACGGTG(+AGGACA)GATCCACATGTAAACTGTTTGGGGGCAAAAG NGG 10 8 17 8 24 0 100 5 10