Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613150943_c3qa AGGCCCCCGGGCAAGCAGGGGGTTGGGGGGAG(+AGGACA)GATCCCGGCAGGCTCAGGTTTTACCCTCTAC NGG 10 8 17 8 24 0 100 5 10
    240613150942_6lwi AGGCAGACAGCACCCTGTGGGAGATCAGATGG(+AGGACA)GATCCTGAGCCCCTTGTATGGCCGTGGGTGG NGG 10 8 17 8 24 0 100 5 10
    240613150940_vokc AGAGGCCACCCACGGCCATACAAGGGGCTCAG(+AGGACA)GATCCCATCTGATCTCCCACAGGGTGCTGTC NGG 10 8 17 8 24 0 100 5 10
    240613150938_hfgs AGGGGCCAACAACACAGTGTAAAGAGACCCAG(+AGGACA)GATCCCTCTGTGATCCCCACCACAATGGAGT NGG 10 8 17 8 24 0 100 5 10
    240613150936_339q TCAGTACTCCATTGTGGTGGGGATCACAGAGG(+AGGACA)GATCCTGGGTCTCTTTACACTGTGTTGTTGG NGG 10 8 17 8 24 0 100 5 10
    240613150935_b7ro ACACACATAGCTAGTTTTGCTTAAAATATTTG(+AGGACA)GATCCCGAGTTTCCATTTTCCTAAAAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240613150934_3edn GGTTTCTCTTTTTTAGGAAAATGGAAACTCGG(+AGGACA)GATCCAAATATTTTAAGCAAAACTAGCTATG NGG 10 8 17 8 24 0 100 5 10
    240613150932_qp2v ACACACATAGCTAGTTTTGCTTAAAATATTTG(+AGGACA)GATCCCGAGTTTCCATTTTCCTAAAAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240613150931_0oqy GGTTTCTCTTTTTTAGGAAAATGGAAACTCGG(+AGGACA)GATCCAAATATTTTAAGCAAAACTAGCTATG NGG 10 8 17 8 24 0 100 5 10
    240613150930_lmw2 GTGCTTAAGTAGCTAGATTCAAATCAAACATG(+AGGACA)GATCCAAAAAAGTCTATTACCAACCAAAGAG NGG 10 8 17 8 24 0 100 5 10