Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613151309_gq9k GTTTCTGGTGTAACTGGGGATGGTGTGAGAAG(+AGGACA)GTACCTGAGAGGACCTTACCTCCAGAGGGAT NGG 10 8 17 8 24 0 100 5 10
    240613151308_25sx AGAAAATCTTGCAAAATACATGGTTGCTTCAG(+AGGACA)GTACCAGCTCTTTACAATACAGTTCAAACTC NGG 10 8 17 8 24 0 100 5 10
    240613151307_2gjd AAAAAGAGTTTGAACTGTATTGTAAAGAGCTG(+AGGACA)GTACCTGAAGCAACCATGTATTTTGCAAGAT NGG 10 8 17 8 24 0 100 5 10
    240613151305_i5s1 CCAAGCTTTTTTTTTTTTAAGGCAAATTTTTG(+AGGACA)GTACCTTTTTTCCTAAACATCTTCGTGAATA NGG 10 8 17 8 24 0 100 5 10
    240613151304_258k TTTAATATTCACGAAGATGTTTAGGAAAAAAG(+AGGACA)GTACCAAAAATTTGCCTTAAAAAAAAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240613151303_upb3 ATGAACACACACATAATCCAGCACGTAGTAGG(+AGGACA)GATCCTCCTCGCCTAGGCTAAATCACTTTAG NGG 10 8 17 8 24 0 100 5 10
    240613151301_4s2o TTCACCTAAAGTGATTTAGCCTAGGCGAGGAG(+AGGACA)GATCCCTACTACGTGCTGGATTATGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240613151300_9q1k CCTACATAAGCCCTTCATTGTGGGAGTAAATG(+AGGACA)GTACCGAAGCCCCAGAAAGTCCTACAGAGAT NGG 10 8 17 8 24 0 100 5 10
    240613151258_71tp AAAAAATCTCTGTAGGACTTTCTGGGGCTTCG(+AGGACA)GTACCATTTACTCCCACAATGAAGGGCTTAT NGG 10 8 17 8 24 0 100 5 10
    240613151257_shdx TGTGATCCCAACACAGGAAGTGGCAACAGGAG(+AGGACA)GATCCAGAGTTCAAAGTCAGCCTTGGATACA NGG 10 8 17 8 24 0 100 5 10