Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613151424_t2ih ATCTAAATATATTTTTCTTTTGTTATTGAAAG(+AGGACA)GTACCAAAGGCTTTTTATGTTTTGTTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240613151423_c11g GAGGCAACTGCAGACGAATCAGAAAATAAGAG(+AGGACA)GATCCACCACCACAGAACTGATTGGCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240613151421_1gt2 ATTGCTCTGAGCCAATCAGTTCTGTGGTGGTG(+AGGACA)GATCCTCTTATTTTCTGATTCGTCTGCAGTT NGG 10 8 17 8 24 0 100 5 10
    240613151420_esuo GTAAGAATAAGTTGATAGCTTCAAATCTACAG(+AGGACA)GATCCCTTCAGCTGGCACTATCTCCCTAAAA NGG 10 8 17 8 24 0 100 5 10
    240613151418_yj6a AGACATTTTAGGGAGATAGTGCCAGCTGAAGG(+AGGACA)GATCCTGTAGATTTGAAGCTATCAACTTATT NGG 10 8 17 8 24 0 100 5 10
    240613151417_fm4k TTACAGGGAAATTCATTATAGATATTCTTTTG(+AGGACA)GTACCTGTGAATTAAATATAAATATAAGATG NGG 10 8 17 8 24 0 100 5 10
    240613151416_h6h1 GAGTTCATCTTATATTTATATTTAATTCACAG(+AGGACA)GTACCAAAAGAATATCTATAATGAATTTCCC NGG 10 8 17 8 24 0 100 5 10
    240613151415_llmp ATACCCCTTGGCTATGCCCACAAATCCATAAG(+AGGACA)GATCCTGACTTAGAAAGAAAAACAGCACAAA NGG 10 8 17 8 24 0 100 5 10
    240613151413_v884 TTGAATTTGTGCTGTTTTTCTTTCTAAGTCAG(+AGGACA)GATCCTTATGGATTTGTGGGCATAGCCAAGG NGG 10 8 17 8 24 0 100 5 10
    240613151412_b9hc AGCAAGGCTCTCTCTTCTGGGCAAGGGCAATG(+AGGACA)GATCCTCAGAGGCCCCGGCAGAAGCCTAGCT NGG 10 8 17 8 24 0 100 5 10