Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613151713_uumm TCCTGAGGGTATCAGAAAGCCCTACACTGTTG(+AGGACA)GTACCTGGGCCCCAACATACTGTGAACGGAA NGG 10 8 17 8 24 0 100 5 10
    240613151712_ycy8 GGGGAGGGAGATGAGGCTGCCCCAAGGCTCTG(+AGGACA)GTACCTGGCTTTAGTTTTCTGTCTTCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240613151710_r8g0 CCTGAGGAAAGAAGACAGAAAACTAAAGCCAG(+AGGACA)GTACCAGAGCCTTGGGGCAGCCTCATCTCCC NGG 10 8 17 8 24 0 100 5 10
    240613151709_s4ma GAGTATTATGGAGCCCGTGGCTTTGTGCTATG(+AGGACA)GATCCTGGGATGGGGGCCTCTTACCCCCCAC NGG 10 8 17 8 24 0 100 5 10
    240613151706_x8n1 GGCTAGTGGGGGGTAAGAGGCCCCCATCCCAG(+AGGACA)GATCCATAGCACAAAGCCACGGGCTCCATAA NGG 10 8 17 8 24 0 100 5 10
    240613151704_tqqr CAGACTGGGTAAGATGTGGCTCTCTCAGGTGG(+AGGACA)GATCCCTTTATTTATTTACTCAAGTCCCCAT NGG 10 8 17 8 24 0 100 5 10
    240613151703_twh8 TTTGTATGGGGACTTGAGTAAATAAATAAAGG(+AGGACA)GATCCCACCTGAGAGAGCCACATCTTACCCA NGG 10 8 17 8 24 0 100 5 10
    240613151702_rqgd TTATTATTCATTTAACCATCCATCCACACAAG(+AGGACA)GTACCTTCTTGAAACCTCTGGGCAGTCTCAC NGG 10 8 17 8 24 0 100 5 10
    240613151700_gvq5 CAAATGTGAGACTGCCCAGAGGTTTCAAGAAG(+AGGACA)GTACCTTGTGTGGATGGATGGTTAAATGAAT NGG 10 8 17 8 24 0 100 5 10
    240613151659_uusc AAGGAACATTTAACATCAAGATTTAAACAGTG(+AGGACA)GTACCACTTGGGTGGCAAACTGGTTCTTAAA NGG 10 8 17 8 24 0 100 5 10