Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613150304_bbnf AATTTGGGTGGATGGGTTACTGCATTCTCAAG(+AGGACA)GATCCATGGCATAGGAAATTGTTCTTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613150302_xfx2 AACTACCCAGAAGAACAATTTCCTATGCCATG(+AGGACA)GATCCTTGAGAATGCAGTAACCCATCCACCC NGG 10 8 17 8 24 0 100 5 10
    240613150300_ep8p GGATGATTCTACCTCCAGACTTCCTCTTCCTG(+AGGACA)GATCCATAGCAATATGCAGCTATGGCCTTGT NGG 10 8 17 8 24 0 100 5 10
    240613150259_mrds TTTATACAAGGCCATAGCTGCATATTGCTATG(+AGGACA)GATCCAGGAAGAGGAAGTCTGGAGGTAGAAT NGG 10 8 17 8 24 0 100 5 10
    240613150258_8ktt TTCCCTTCAGTATCTGCTCAGGCAGAAGATGG(+AGGACA)GTACCCCCACCTCCACCATCATCTTCTGCTC NGG 10 8 17 8 24 0 100 5 10
    240613150256_j1ym CGTGGGAGCAGAAGATGATGGTGGAGGTGGGG(+AGGACA)GTACCCATCTTCTGCCTGAGCAGATACTGAA NGG 10 8 17 8 24 0 100 5 10
    240613150254_vmts ATGTTCTGGGATGCCTCCCCTGAAGCCAATGG(+AGGACA)GTACCAACATCACCAGGAGGAGGAAGCAAAA NGG 10 8 17 8 24 0 100 5 10
    240613150253_1guz GACTCTTTTGCTTCCTCCTCCTGGTGATGTTG(+AGGACA)GTACCCATTGGCTTCAGGGGAGGCATCCCAG NGG 10 8 17 8 24 0 100 5 10
    240613150251_i7ag AGTCTGTGAGGGAGCCTGACAGGGAGCACCAG(+AGGACA)GATCCTTCACCTCCCCACTGCCGTGTGTCTA NGG 10 8 17 8 24 0 100 5 10
    240613150250_1q8e GTGTGTAGACACACGGCAGTGGGGAGGTGAAG(+AGGACA)GATCCTGGTGCTCCCTGTCAGGCTCCCTCAC NGG 10 8 17 8 24 0 100 5 10