Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613150205_f64n CTCATGGAAGCTATTTTGCTCTGTACCCCGGG(+AGGACA)GATCCCCAGCATTAGGCCATTGGACCCCATC NGG 10 8 17 8 24 0 100 5 10
    240613150203_ch8b AGAGAGATGGGGTCCAATGGCCTAATGCTGGG(+AGGACA)GATCCCCGGGGTACAGAGCAAAATAGCTTCC NGG 10 8 17 8 24 0 100 5 10
    240613150202_4ok7 AAGCTAAATGTGTGCATACTCTATGTGGAGTG(+AGGACA)GATCCACTCCGAACTGTACACCCTAAATACA NGG 10 8 17 8 24 0 100 5 10
    240613150201_8ikw GCACATGTATTTAGGGTGTACAGTTCGGAGTG(+AGGACA)GATCCACTCCACATAGAGTATGCACACATTT NGG 10 8 17 8 24 0 100 5 10
    240613150159_3l0r GGGCTCTGCTCAGCATTGTCTGAGGGGTTGGG(+AGGACA)GTACCCACACAGGTGCTTGCTGCTTCCCACG NGG 10 8 17 8 24 0 100 5 10
    240613150157_mlwf AATGCCGTGGGAAGCAGCAAGCACCTGTGTGG(+AGGACA)GTACCCCAACCCCTCAGACAATGCTGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240613150155_82ry CACCAAGCCTGATGACCTGAGTTAGACCTCGG(+AGGACA)GATCCATGAGGTAGAAGAAGAGAAATGAGCC NGG 10 8 17 8 24 0 100 5 10
    240613150153_cwck TAAAGGGCTCATTTCTCTTCTTCTACCTCATG(+AGGACA)GATCCCGAGGTCTAACTCAGGTCATCAGGCT NGG 10 8 17 8 24 0 100 5 10
    240613150152_17th GAGGTTCTGGGTTCAATTCCCATCTGTAATGG(+AGGACA)GATCCGATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613150150_189n GCTGTCTTCAGACACACCAGAAGAGGGCATCG(+AGGACA)GATCCCATTACAGATGGGAATTGAACCCAGA NGG 10 8 17 8 24 0 100 5 10