Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613150359_2ibx CCAATAATGAAGTCCCAGATATCAGAAAACTG(+AGGACA)GATCCTGGCCTGAGTAGGGGGTGATACACAG NGG 10 8 17 8 24 0 100 5 10
    240613150357_zo81 CCATTCTGTGTATCACCCCCTACTCAGGCCAG(+AGGACA)GATCCAGTTTTCTGATATCTGGGACTTCATT NGG 10 8 17 8 24 0 100 5 10
    240613150356_kic1 TCACCTTATTTTTGTAGTCTCTCCCTGAACTG(+AGGACA)GTACCTCACCCATTCAGCTGTCCAGAAAACT NGG 10 8 17 8 24 0 100 5 10
    240613150355_6hmw TCTCAAGTTTTCTGGACAGCTGAATGGGTGAG(+AGGACA)GTACCAGTTCAGGGAGAGACTACAAAAATAA NGG 10 8 17 8 24 0 100 5 10
    240613150354_i9ep AGATGGGGTCTGTGATGGATTGTTACACTGTG(+AGGACA)GATCCCTGCACTTGGCATCTGCACGAGTCTA NGG 10 8 17 8 24 0 100 5 10
    240613150352_o7xm TGGCCTAGACTCGTGCAGATGCCAAGTGCAGG(+AGGACA)GATCCACAGTGTAACAATCCATCACAGACCC NGG 10 8 17 8 24 0 100 5 10
    240613150351_d9hw AATGTACAGTTGCCTAACAGACAACTTCTAGG(+AGGACA)GATCCTACAAATTCCCAGGGGATCTGTACTC NGG 10 8 17 8 24 0 100 5 10
    240613150349_umef ACCTAGAGTACAGATCCCCTGGGAATTTGTAG(+AGGACA)GATCCCTAGAAGTTGTCTGTTAGGCAACTGT NGG 10 8 17 8 24 0 100 5 10
    240613150347_d3us GGATGGACTATCCAGAGACTACCCCACCCAGG(+AGGACA)GATCCATCCCATAATCAGCCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240613150345_kco8 AGTGTCTGGGTTTGGTGGCTGATTATGGGATG(+AGGACA)GATCCCTGGGTGGGGTAGTCTCTGGATAGTC NGG 10 8 17 8 24 0 100 5 10