Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613150102_2ii3 GAACTTGCTGCTAAGCCAGACAGCCTGAGTTG(+AGGACA)GATCCCTGGGACTCATGGCCTGCTGCCGGCT NGG 10 8 17 8 24 0 100 5 10
    240613150059_4eyw AAGACAGCCGGCAGCAGGCCATGAGTCCCAGG(+AGGACA)GATCCAACTCAGGCTGTCTGGCTTAGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240613150058_bbmb TGGACCCCCAAAAGTTCATGCACTGACGGCTG(+AGGACA)GATCCTCGGGCCGTAGAGTTCAGTGTAGGAT NGG 10 8 17 8 24 0 100 5 10
    240613150055_d7j9 CTCCCATCCTACACTGAACTCTACGGCCCGAG(+AGGACA)GATCCAGCCGTCAGTGCATGAACTTTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240613150054_g1do ATCCTGATTTAGAATAATTGACACTGTTTTAG(+AGGACA)GTACCCGTTTTCTATTACCTTAAACTATTCA NGG 10 8 17 8 24 0 100 5 10
    240613150052_nkyf GATACTGAATAGTTTAAGGTAATAGAAAACGG(+AGGACA)GTACCTAAAACAGTGTCAATTATTCTAAATC NGG 10 8 17 8 24 0 100 5 10
    240613150051_9itd TAAAGAAAATACAGAACTCCACAGAAAGCTTG(+AGGACA)GTACCATCTTCCAGCTAACACAGTATAGAGC NGG 10 8 17 8 24 0 100 5 10
    240613150050_2cxy CATTGGCTCTATACTGTGTTAGCTGGAAGATG(+AGGACA)GTACCAAGCTTTCTGTGGAGTTCTGTATTTT NGG 10 8 17 8 24 0 100 5 10
    240613150047_o39o GACAGCTGGAGGAATGAGCCTTCACTGCCCTG(+AGGACA)GTACCACTCTAGGCAACTGCATCTGAGACAT NGG 10 8 17 8 24 0 100 5 10
    240613150046_5vwy GGAACATGTCTCAGATGCAGTTGCCTAGAGTG(+AGGACA)GTACCAGGGCAGTGAAGGCTCATTCCTCCAG NGG 10 8 17 8 24 0 100 5 10