Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613150045_sk56 CTCCTTGGCCATAGGAAAGATGCAACTCACAG(+AGGACA)GTACCAGCTGACATTACAGAGATCAGGACAG NGG 10 8 17 8 24 0 100 5 10
    240613150044_9kc0 TGCAACTGTCCTGATCTCTGTAATGTCAGCTG(+AGGACA)GTACCTGTGAGTTGCATCTTTCCTATGGCCA NGG 10 8 17 8 24 0 100 5 10
    240613150042_c61q TGCTCAGTCTATGATGCCCTTGAGCTACCATG(+AGGACA)GTACCTTGTGCAACATGGCTTGATTAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240613150041_l9n7 CCCAGGAAGCTAATCAAGCCATGTTGCACAAG(+AGGACA)GTACCATGGTAGCTCAAGGGCATCATAGACT NGG 10 8 17 8 24 0 100 5 10
    240613150039_rmi1 TAGTTCTAACTTAAACCACTTGGTGATTGGTG(+AGGACA)GTACCGTCAGGTGCTGGGCTTCAAAAACTTG NGG 10 8 17 8 24 0 100 5 10
    240613150038_tgjz CTTGCCAAGTTTTTGAAGCCCAGCACCTGACG(+AGGACA)GTACCACCAATCACCAAGTGGTTTAAGTTAG NGG 10 8 17 8 24 0 100 5 10
    240613150037_3ja4 GTGTCAAATTCTGTGAACCAGGGCCAACACTG(+AGGACA)GTACCAGCTTCACCCTGACCTGGGGCCACTG NGG 10 8 17 8 24 0 100 5 10
    240613150035_rg3k GAGGGCAGTGGCCCCAGGTCAGGGTGAAGCTG(+AGGACA)GTACCAGTGTTGGCCCTGGTTCACAGAATTT NGG 10 8 17 8 24 0 100 5 10
    240613150034_8ohh TATAGCTAAGTTTACACCAACCAAATTATTTG(+AGGACA)GTACCAATTCTTGTAAATACTGGGTATGCTG NGG 10 8 17 8 24 0 100 5 10
    240613150033_ek1f TACCCCAGCATACCCAGTATTTACAAGAATTG(+AGGACA)GTACCAAATAATTTGGTTGGTGTAAACTTAG NGG 10 8 17 8 24 0 100 5 10