Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613150019_m5m0 ATTGCAGATTATGCACAAGGACCACAGTTCAG(+AGGACA)GTACCAAATCTCAAGTGGAAGTCAGATTATT NGG 10 8 17 8 24 0 100 5 10
    240613150017_yyv8 GACACAATAATCTGACTTCCACTTGAGATTTG(+AGGACA)GTACCTGAACTGTGGTCCTTGTGCATAATCT NGG 10 8 17 8 24 0 100 5 10
    240613150016_6m60 ATCTATAGTCCTAGGGCTAGGGAGACAGAGAG(+AGGACA)GATCCCTGGAGCATGGTGGCAGATGAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240613150014_tmso TCATTACTCTTCATCTGCCACCATGCTCCAGG(+AGGACA)GATCCTCTCTGTCTCCCTAGCCCTAGGACTA NGG 10 8 17 8 24 0 100 5 10
    240613150013_1dub GAGCTGCAGGGAGCCACTCTGAGTCTGTGAGG(+AGGACA)GATCCACAGAGCCTAAGATGTGCACGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240613150011_6vpi GAATTTCAGGCGTGCACATCTTAGGCTCTGTG(+AGGACA)GATCCCTCACAGACTCAGAGTGGCTCCCTGC NGG 10 8 17 8 24 0 100 5 10
    240613150010_8oed GAAACCAGTGGCTCTGGTACTCAGTAGTTAGG(+AGGACA)GTACCCTGTGCTTCTTGGTGCTTTTCCTTTC NGG 10 8 17 8 24 0 100 5 10
    240613150008_5k7f TTCCAGAAAGGAAAAGCACCAAGAAGCACAGG(+AGGACA)GTACCCTAACTACTGAGTACCAGAGCCACTG NGG 10 8 17 8 24 0 100 5 10
    240613150007_ykrl TCCCACACTATTAGCTGCTATCAGAAAAACAG(+AGGACA)GATCCTACAAGTCAAGGTTACTATGTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240613150006_7rp3 TAATTAGGAAACATAGTAACCTTGACTTGTAG(+AGGACA)GATCCTGTTTTTCTGATAGCAGCTAATAGTG NGG 10 8 17 8 24 0 100 5 10