Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613145917_7c2h AGTAGCTGAAAAAAACCAGACAGACAGAGCAG(+AGGACA)GTACCAGGACTGACTACAGCTCAGGAGTCCA NGG 10 8 17 8 24 0 100 5 10
    240613145915_sbpp TAAGATGGACTCCTGAGCTGTAGTCAGTCCTG(+AGGACA)GTACCTGCTCTGTCTGTCTGGTTTTTTTCAG NGG 10 8 17 8 24 0 100 5 10
    240613145914_23c2 GGAAGTAAGTCTTGCTGAAAAGCCACACCAAG(+AGGACA)GATCCTAGCAGTAGGCGGGTAAGTGACTCCA NGG 10 8 17 8 24 0 100 5 10
    240613145912_dinu GGGGCTGGAGTCACTTACCCGCCTACTGCTAG(+AGGACA)GATCCTTGGTGTGGCTTTTCAGCAAGACTTA NGG 10 8 17 8 24 0 100 5 10
    240613145910_2xpl TGCCCATGGTCACTGACCTGAGAGGGGTTAGG(+AGGACA)GTACCTTTCACAGAAGACAGGGCTCAGTAAA NGG 10 8 17 8 24 0 100 5 10
    240613145909_aryy ACCACTTTACTGAGCCCTGTCTTCTGTGAAAG(+AGGACA)GTACCCTAACCCCTCTCAGGTCAGTGACCAT NGG 10 8 17 8 24 0 100 5 10
    240613145908_3j95 TTCTCGGTTAAGGTTGGACAGTCATTTCATTG(+AGGACA)GTACCAGACCACATTTGCAGCAAGATTTCAC NGG 10 8 17 8 24 0 100 5 10
    240613145907_ub4h ACAAGGTGAAATCTTGCTGCAAATGTGGTCTG(+AGGACA)GTACCAATGAAATGACTGTCCAACCTTAACC NGG 10 8 17 8 24 0 100 5 10
    240613145905_nv43 AAGAGCCACACCCAACAAGACATGGGGTAGGG(+AGGACA)GATCCTGTGTCATACTTGAGGCTCTGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240613145904_l3o1 GCGCCAAAGCCAGAGCCTCAAGTATGACACAG(+AGGACA)GATCCCCTACCCCATGTCTTGTTGGGTGTGG NGG 10 8 17 8 24 0 100 5 10