Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613145847_eorv TCCCCAGAGCCTGATACAGGTGACCTCGGAAG(+AGGACA)GTACCAGCAGTAGGACCAGTGGACCGTGCTC NGG 10 8 17 8 24 0 100 5 10
    240613145846_p59o AAGCGGAGCACGGTCCACTGGTCCTACTGCTG(+AGGACA)GTACCTTCCGAGGTCACCTGTATCAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240613145844_rwah ATGGAACCTTAACTGGGAGCTGCCGGATAAAG(+AGGACA)GTACCCTTGCTCTCTGTGCTTATGGTTTGTC NGG 10 8 17 8 24 0 100 5 10
    240613145843_09ce TAGGTGACAAACCATAAGCACAGAGAGCAAGG(+AGGACA)GTACCTTTATCCGGCAGCTCCCAGTTAAGGT NGG 10 8 17 8 24 0 100 5 10
    240613145842_rxoh GAGGCTCCTGAATTGTTTATTGTGTAGACATG(+AGGACA)GATCCTGTGAAGGAAAAACTTAAACCCGAGG NGG 10 8 17 8 24 0 100 5 10
    240613145841_bpf1 ATTACCCTCGGGTTTAAGTTTTTCCTTCACAG(+AGGACA)GATCCATGTCTACACAATAAACAATTCAGGA NGG 10 8 17 8 24 0 100 5 10
    240613145839_vcox TTTCTAAGTCAGGTAGCTAAGACTGTCTCCTG(+AGGACA)GTACCCGGTGTACATTCTTGGGATCCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240613145837_frsy AACAAGAGAAGGATCCCAAGAATGTACACCGG(+AGGACA)GTACCAGGAGACAGTCTTAGCTACCTGACTT NGG 10 8 17 8 24 0 100 5 10
    240613145835_8onm ACTGTCTCCTGGTACCCGGTGTACATTCTTGG(+AGGACA)GATCCTTCTCTTGTTGGCCTATCCGTTAGTT NGG 10 8 17 8 24 0 100 5 10
    240613145834_rfx4 TGCTGAACTAACGGATAGGCCAACAAGAGAAG(+AGGACA)GATCCCAAGAATGTACACCGGGTACCAGGAG NGG 10 8 17 8 24 0 100 5 10