Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613145742_zyi6 GCAGGTCGGGGTTCTCTCAAGTCCCCCAGTCG(+AGGACA)GATCCGGGACCCCTTTCTGGGCTAGGAAGCG NGG 10 8 17 8 24 0 100 5 10
    240613145740_l9yl GCGGGCGCTTCCTAGCCCAGAAAGGGGTCCCG(+AGGACA)GATCCGACTGGGGGACTTGAGAGAACCCCGA NGG 10 8 17 8 24 0 100 5 10
    240613145738_hb93 CTGGAGGGATATAAAGGGAACACAGACTTGAG(+AGGACA)GTACCACAGGCTTTGTCTGAAGAGAGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240613145736_mdrd GTGACAGGGACTCTCTTCAGACAAAGCCTGTG(+AGGACA)GTACCTCAAGTCTGTGTTCCCTTTATATCCC NGG 10 8 17 8 24 0 100 5 10
    240613145734_d4xd GGTGACCCAAGCCCAGTGACATCCCTTCAGGG(+AGGACA)GATCCATGTGAGCCTTCCAGGTACTAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240613145733_czne CCGCTGTTTCTAGTACCTGGAAGGCTCACATG(+AGGACA)GATCCCCTGAAGGGATGTCACTGGGCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240613145732_0izj TGACAGCTCACAACTGTCTGAACCCTTCACTG(+AGGACA)GTACCAGGCACCATGGGGTGCACAGAGAGAT NGG 10 8 17 8 24 0 100 5 10
    240613145730_1ouy CATGTATCTCTCTGTGCACCCCATGGTGCCTG(+AGGACA)GTACCAGTGAAGGGTTCAGACAGTTGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240613145728_7b6w TGGCCAGGCTCAAGCTGGGGTTTTCCAACTCG(+AGGACA)GATCCAGTCAAGAAAAATCCCTCACAGGTGT NGG 10 8 17 8 24 0 100 5 10
    240613145727_afyj TGGGCACACCTGTGAGGGATTTTTCTTGACTG(+AGGACA)GATCCGAGTTGGAAAACCCCAGCTTGAGCCT NGG 10 8 17 8 24 0 100 5 10