Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613145758_qwsa GTCAGCTGATCCGCCGTGCCTGTGGACCCCTG(+AGGACA)GATCCTTGTACTTTGTACACGTGCCACACAC NGG 10 8 17 8 24 0 100 5 10
    240613145757_jwke CCAGGGTGTGTGGCACGTGTACAAAGTACAAG(+AGGACA)GATCCAGGGGTCCACAGGCACGGCGGATCAG NGG 10 8 17 8 24 0 100 5 10
    240613145755_rqa2 AAGGAGAGTTGGTCTCAGAATCCCCAGGCTGG(+AGGACA)GATCCTGGCTAGCTGGCCCCTTGTCATCAAG NGG 10 8 17 8 24 0 100 5 10
    240613145753_owfr TCCTGCTTGATGACAAGGGGCCAGCTAGCCAG(+AGGACA)GATCCCAGCCTGGGGATTCTGAGACCAACTC NGG 10 8 17 8 24 0 100 5 10
    240613145752_8bkl CACAATGGCAGCTTGTAGCCTTCACCCTTCTG(+AGGACA)GTACCAGGACTTCATCAACTGAACATTGTTA NGG 10 8 17 8 24 0 100 5 10
    240613145750_5vl0 ATGTATAACAATGTTCAGTTGATGAAGTCCTG(+AGGACA)GTACCAGAAGGGTGAAGGCTACAAGCTGCCA NGG 10 8 17 8 24 0 100 5 10
    240613145747_kjjq TGCTGAACCTCACTCTCCCTTCAACCTCCCCG(+AGGACA)GATCCAAACCAGCTCCAGGAGGAGGGACAGG NGG 10 8 17 8 24 0 100 5 10
    240613145746_bk7o CACCGCCTGTCCCTCCTCCTGGAGCTGGTTTG(+AGGACA)GATCCGGGGAGGTTGAAGGGAGAGTGAGGTT NGG 10 8 17 8 24 0 100 5 10
    240613145745_7yho ACAGGTCCGTCCCGGAGCACACAGAGGTGTTG(+AGGACA)GTACCAAGACTGAAACCTTCACAGATTCTAT NGG 10 8 17 8 24 0 100 5 10
    240613145743_jee8 GCCCAATAGAATCTGTGAAGGTTTCAGTCTTG(+AGGACA)GTACCAACACCTCTGTGTGCTCCGGGACGGA NGG 10 8 17 8 24 0 100 5 10