Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613145650_yggo TTCATTTATCCCATTATAAAACGGAGGTCAGG(+AGGACA)GATCCCTCCGGGTATCTAACCACGGTCAACT NGG 10 8 17 8 24 0 100 5 10
    240613145648_7trk GGAAGAGTTGACCGTGGTTAGATACCCGGAGG(+AGGACA)GATCCCTGACCTCCGTTTTATAATGGGATAA NGG 10 8 17 8 24 0 100 5 10
    240613145646_y3e7 GCCAGATTTTCACGGGAAGGTCTTTAGTTCCG(+AGGACA)GATCCTTGTAAGTATGAGTCCTTCATAGGGT NGG 10 8 17 8 24 0 100 5 10
    240613145645_3ura ATATAACCCTATGAAGGACTCATACTTACAAG(+AGGACA)GATCCGGAACTAAAGACCTTCCCGTGAAAAT NGG 10 8 17 8 24 0 100 5 10
    240613145643_0q6r GGATGCTCACCCAACATCTGCTTTCCTGGTTG(+AGGACA)GATCCAGATTTGCACCATTTAAACATTGATG NGG 10 8 17 8 24 0 100 5 10
    240613145642_ct2v ATGCTCATCAATGTTTAAATGGTGCAAATCTG(+AGGACA)GATCCAACCAGGAAAGCAGATGTTGGGTGAG NGG 10 8 17 8 24 0 100 5 10
    240613145641_mrcd TTCTGCCTCTTTTCATAGTTCTGATGCACACG(+AGGACA)GTACCGGCTGGTTTTGTGCGTCAACCTGACA NGG 10 8 17 8 24 0 100 5 10
    240613145639_91qh GCTTGTGTCAGGTTGACGCACAAAACCAGCCG(+AGGACA)GTACCGTGTGCATCAGAACTATGAAAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240613145638_ijdy CTCAGGAATGGGATTGGTGTGATGCCATAATG(+AGGACA)GTACCTGCCCTGTCCAAGGACAGCCTGGGTC NGG 10 8 17 8 24 0 100 5 10
    240613145636_96i3 TGTATGACCCAGGCTGTCCTTGGACAGGGCAG(+AGGACA)GTACCATTATGGCATCACACCAATCCCATTC NGG 10 8 17 8 24 0 100 5 10