Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613145601_r6p9 TGAACATCATAGCCTTGCCGTTAAGTCCCCAG(+AGGACA)GTACCTCCAACCTCCCTGCTGTCATGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240613145559_rwel CACACATGCCCATGACAGCAGGGAGGTTGGAG(+AGGACA)GTACCTGGGGACTTAACGGCAAGGCTATGAT NGG 10 8 17 8 24 0 100 5 10
    240613145557_h4s9 CGGGTGGCCAAGAACGACGGGGTGCCTCCTAG(+AGGACA)GATCCCAGGACTTACTGGGGCTTTTCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240613145555_3ahj CAGCACAACTGAAAAGCCCCAGTAAGTCCTGG(+AGGACA)GATCCTAGGAGGCACCCCGTCGTTCTTGGCC NGG 10 8 17 8 24 0 100 5 10
    240613145554_r7iq AAACCAGTCTGTGATGCAGGATGGTTGGCTCG(+AGGACA)GATCCCGAGGGCGGGCCTATGAGAGAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240613145552_tc0v AGTGCTCACCTCTCTCATAGGCCCGCCCTCGG(+AGGACA)GATCCGAGCCAACCATCCTGCATCACAGACT NGG 10 8 17 8 24 0 100 5 10
    240613145550_7epn TGTGAGGGAAGTCGCTTCCCGCGAGATGCTGG(+AGGACA)GATCCAGCCTGGTTGTCTTTCCGTAAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240613145548_vp9b CACAGCCACTTTACGGAAAGACAACCAGGCTG(+AGGACA)GATCCCAGCATCTCGCGGGAAGCGACTTCCC NGG 10 8 17 8 24 0 100 5 10
    240613145547_vghw TTTTCCCATCCCAGCTTCCTTCTGGTAAACTG(+AGGACA)GTACCCTCAGGATAAAGAAGTATATGGTGCC NGG 10 8 17 8 24 0 100 5 10
    240613145545_pmqq AGGCAGGCACCATATACTTCTTTATCCTGAGG(+AGGACA)GTACCAGTTTACCAGAAGGAAGCTGGGATGG NGG 10 8 17 8 24 0 100 5 10