Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613145513_64i4 ACCCATTTAATCTCAGCACTTCAGAATCAATG(+AGGACA)GATCCTTGAGTTTGAGGCCAGCCTGGGTACA NGG 10 8 17 8 24 0 100 5 10
    240613145512_q1p9 TAGAATGTACCCAGGCTGGCCTCAAACTCAAG(+AGGACA)GATCCATTGATTCTGAAGTGCTGAGATTAAA NGG 10 8 17 8 24 0 100 5 10
    240613145510_pce4 GACCTCAAGGACAACTCCTGAAAGCCAGCTGG(+AGGACA)GATCCCAGGAACAGCAGTGAGACCCGGTCAT NGG 10 8 17 8 24 0 100 5 10
    240613145508_gm32 GTGGGATGACCGGGTCTCACTGCTGTTCCTGG(+AGGACA)GATCCCAGCTGGCTTTCAGGAGTTGTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240613145507_5l4x TCAGAGGTGGGACAGAGAGCTCGGCCACTCAG(+AGGACA)GATCCATCATGCAAGCAAGCAGGGTTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240613145506_5mjw CGGCCGGAAGAACCCTGCTTGCTTGCATGATG(+AGGACA)GATCCTGAGTGGCCGAGCTCTCTGTCCCACC NGG 10 8 17 8 24 0 100 5 10
    240613145504_nh68 CCTGGAAACAACCAGGATCCTATCCATTCAAG(+AGGACA)GTACCATGGGGCAGGTCCAATCTTCAAACCA NGG 10 8 17 8 24 0 100 5 10
    240613145502_xklh GCCATTGGTTTGAAGATTGGACCTGCCCCATG(+AGGACA)GTACCTTGAATGGATAGGATCCTGGTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240613145501_h48d TTCAACTTTTAAAACAGAAGAGGCGTTGCTAG(+AGGACA)GATCCCAGACTCTCTGACATCTAAGGCCGAC NGG 10 8 17 8 24 0 100 5 10
    240613145459_f3os CCTGTGTCGGCCTTAGATGTCAGAGAGTCTGG(+AGGACA)GATCCTAGCAACGCCTCTTCTGTTTTAAAAG NGG 10 8 17 8 24 0 100 5 10