Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613145458_rbub GGTTATAAAGACTGCTATCACCTTTGGCAGTG(+AGGACA)GTACCAAGGAACAGTATGTATGAATGTGATG NGG 10 8 17 8 24 0 100 5 10
    240613145456_ms7j AAGATCATCACATTCATACATACTGTTCCTTG(+AGGACA)GTACCACTGCCAAAGGTGATAGCAGTCTTTA NGG 10 8 17 8 24 0 100 5 10
    240613145455_f077 ACAATAAGATCCCAAAGACACAGCAACATTCG(+AGGACA)GATCCACGAGTTAGAGGACTTTCATGCCTAG NGG 10 8 17 8 24 0 100 5 10
    240613145453_2s8m AGGATCTAGGCATGAAAGTCCTCTAACTCGTG(+AGGACA)GATCCGAATGTTGCTGTGTCTTTGGGATCTT NGG 10 8 17 8 24 0 100 5 10
    240613145451_acv0 GCCTGTGTTTCTTTTTAAAATGTTAAGGCCTG(+AGGACA)GTACCTTTATTATTTATGACTCCTGGGCGAT NGG 10 8 17 8 24 0 100 5 10
    240613145450_8j66 CCTGAATCGCCCAGGAGTCATAAATAATAAAG(+AGGACA)GTACCAGGCCTTAACATTTTAAAAAGAAACA NGG 10 8 17 8 24 0 100 5 10
    240613145449_4g0t AGCTTTAGTAAAGACAGTCCCCACCATCAAGG(+AGGACA)GATCCAACTACCTAGAGTAACAGCATTAAGT NGG 10 8 17 8 24 0 100 5 10
    240613145447_kkzj TGAAGACTTAATGCTGTTACTCTAGGTAGTTG(+AGGACA)GATCCCTTGATGGTGGGGACTGTCTTTACTA NGG 10 8 17 8 24 0 100 5 10
    240613145446_jmlf GGAAACCAAGCTTGGGCCTTGCAGTGGAAATG(+AGGACA)GATCCGAGGGCCCAGGGGACCCTGGTGGATT NGG 10 8 17 8 24 0 100 5 10
    240613145444_komp CCCTAAATCCACCAGGGTCCCCTGGGCCCTCG(+AGGACA)GATCCATTTCCACTGCAAGGCCCAAGCTTGG NGG 10 8 17 8 24 0 100 5 10