Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613145529_2svl GACGCCATTGGAGTAGATCACCGCAGGTGACG(+AGGACA)GTACCAGCCTGAAGCCCCACCCAAAAGGCGC NGG 10 8 17 8 24 0 100 5 10
    240613145527_f3r9 GGTAGGCGCCTTTTGGGTGGGGCTTCAGGCTG(+AGGACA)GTACCGTCACCTGCGGTGATCTACTCCAATG NGG 10 8 17 8 24 0 100 5 10
    240613145526_ftu5 GTAATTTTCATAATTGCTAAATGGAATTCATG(+AGGACA)GATCCCATACAATGCCGCCCAACACTTGAGC NGG 10 8 17 8 24 0 100 5 10
    240613145525_mz2z TCCAAGCTCAAGTGTTGGGCGGCATTGTATGG(+AGGACA)GATCCATGAATTCCATTTAGCAATTATGAAA NGG 10 8 17 8 24 0 100 5 10
    240613145523_154u TGGATGAGGAGTGTGTCGCTATAACCACTCTG(+AGGACA)GTACCCAAGAGGGGCTGGGCACCTACTCGGG NGG 10 8 17 8 24 0 100 5 10
    240613145522_ma5x AGTGTCCCGAGTAGGTGCCCAGCCCCTCTTGG(+AGGACA)GTACCAGAGTGGTTATAGCGACACACTCCTC NGG 10 8 17 8 24 0 100 5 10
    240613145520_u14t GACAGCTCTGTCTGTCTGTCTGTCTCTCTTTG(+AGGACA)GTACCAGGGATTACACCTACCTTGGGCCTTG NGG 10 8 17 8 24 0 100 5 10
    240613145518_cvad ACACACAAGGCCCAAGGTAGGTGTAATCCCTG(+AGGACA)GTACCAAAGAGAGACAGACAGACAGACAGAG NGG 10 8 17 8 24 0 100 5 10
    240613145516_bfyl GCTCAGCTCAGCGAGGAGTCTGTAGCCTAGGG(+AGGACA)GATCCAGTTCTGCTCTCAGGCATTAAAAACA NGG 10 8 17 8 24 0 100 5 10
    240613145515_q9eq TTTTTTGTTTTTAATGCCTGAGAGCAGAACTG(+AGGACA)GATCCCCTAGGCTACAGACTCCTCGCTGAGC NGG 10 8 17 8 24 0 100 5 10