Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613145616_mn1x ACCACATGGTGGCTCACAACCATCTGTAATGG(+AGGACA)GATCCTATGCCACCTTCTGGTGTCTGAAGAG NGG 10 8 17 8 24 0 100 5 10
    240613145615_lr2p GGGTGGGGGCGGGATAGGGGCAGTTAAACAAG(+AGGACA)GTACCCTGGGGAAGAAATGAAGGTCAGATCC NGG 10 8 17 8 24 0 100 5 10
    240613145612_6ifg CCATTGGATCTGACCTTCATTTCTTCCCCAGG(+AGGACA)GTACCTTGTTTAACTGCCCCTATCCCGCCCC NGG 10 8 17 8 24 0 100 5 10
    240613145611_4yi9 TTTCAGGACACCTTGTGTCATGTGTGTTCAGG(+AGGACA)GATCCTAACTTGGATCTTCATGATTGCAAAG NGG 10 8 17 8 24 0 100 5 10
    240613145609_zxyl TCTGACTTTGCAATCATGAAGATCCAAGTTAG(+AGGACA)GATCCCTGAACACACATGACACAAGGTGTCC NGG 10 8 17 8 24 0 100 5 10
    240613145608_c4wl AACCTTCCATAGTCTGAGCTTTACAGGAGCAG(+AGGACA)GATCCCCAGCCATCTTCCCTCACTCCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240613145606_ydys TCACACTGGAGGAGTGAGGGAAGATGGCTGGG(+AGGACA)GATCCTGCTCCTGTAAAGCTCAGACTATGGA NGG 10 8 17 8 24 0 100 5 10
    240613145605_s95c TTAGGTCAAATTGAACCTAGGCAAGGACAAAG(+AGGACA)GTACCTTCAGTGAGGAGACCAGGACGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240613145604_4tnc AGTTTCCCTCCGTCCTGGTCTCCTCACTGAAG(+AGGACA)GTACCTTTGTCCTTGCCTAGGTTCAATTTGA NGG 10 8 17 8 24 0 100 5 10
    240613145601_r6p9 TGAACATCATAGCCTTGCCGTTAAGTCCCCAG(+AGGACA)GTACCTCCAACCTCCCTGCTGTCATGGGCAT NGG 10 8 17 8 24 0 100 5 10