Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613145414_sxey GGGGTCAAGTTGGTCACAGGTAGGAATCTGGG(+AGGACA)GTACCATCAGGGTCTAGTGAATGAAGGATTC NGG 10 8 17 8 24 0 100 5 10
    240613145412_gise AGGCTGAATCCTTCATTCACTAGACCCTGATG(+AGGACA)GTACCCCAGATTCCTACCTGTGACCAACTTG NGG 10 8 17 8 24 0 100 5 10
    240613145411_2uoo GTTTGAAAGGTGAACTGTAAGACTCTTCACTG(+AGGACA)GATCCTTAATGTTTCAGTGGTCCAGTCTTCA NGG 10 8 17 8 24 0 100 5 10
    240613145410_k1en TTTGTTGAAGACTGGACCACTGAAACATTAAG(+AGGACA)GATCCAGTGAAGAGTCTTACAGTTCACCTTT NGG 10 8 17 8 24 0 100 5 10
    240613145408_uh9a GGAAGGGCTATCCAGAGACTGCTCCACTTGGG(+AGGACA)GATCCATCCCATATCCAGTCACCAAATCCAG NGG 10 8 17 8 24 0 100 5 10
    240613145406_xh8y AGTGTCTGGATTTGGTGACTGGATATGGGATG(+AGGACA)GATCCCCAAGTGGAGCAGTCTCTGGATAGCC NGG 10 8 17 8 24 0 100 5 10
    240613145404_tn10 AACAAACAAACAAACAAACATATTATTAGAGG(+AGGACA)GATCCTACCAAGGCCTTCTGGTCAACTCTAC NGG 10 8 17 8 24 0 100 5 10
    240613145402_ukz0 CAAGAGTAGAGTTGACCAGAAGGCCTTGGTAG(+AGGACA)GATCCCTCTAATAATATGTTTGTTTGTTTGT NGG 10 8 17 8 24 0 100 5 10
    240613145400_1ku8 TACATATGCAGCTAGAGACACAAACTCAGGGG(+AGGACA)GTACCTGTTAGTTCATATTGTTGTTCCTCCT NGG 10 8 17 8 24 0 100 5 10
    240613145359_fa5q CCTATAGGAGGAACAACAATATGAACTAACAG(+AGGACA)GTACCCCCTGAGTTTGTGTCTCTAGCTGCAT NGG 10 8 17 8 24 0 100 5 10