Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613145308_42se TCACAAACATTTGCCACTGGCCATTGTCTCTG(+AGGACA)GATCCCCTCAGAAAGCTGCCTCCCCTAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613145307_jcmi AGAACACAGCCTGTGCCTGAATTAATGGCTGG(+AGGACA)GATCCATGGATGGAACACTTGGGTATGCACA NGG 10 8 17 8 24 0 100 5 10
    240613145305_mfrp GGCTTTGTGCATACCCAAGTGTTCCATCCATG(+AGGACA)GATCCCAGCCATTAATTCAGGCACAGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240613145303_dmpe TGGGTTTGTGGCCACTCCAGGGGTTTACCCTG(+AGGACA)GATCCTCTGCCTCCGAGGGGCTTGTCCTAAT NGG 10 8 17 8 24 0 100 5 10
    240613145302_q240 GAAAGATTAGGACAAGCCCCTCGGAGGCAGAG(+AGGACA)GATCCAGGGTAAACCCCTGGAGTGGCCACAA NGG 10 8 17 8 24 0 100 5 10
    240613145300_dvf7 TCACTTGTGGGTAGAGGGCAGCTCTCTCGGTG(+AGGACA)GATCCCCACAGCCAATCAAGCTGTAATCGCA NGG 10 8 17 8 24 0 100 5 10
    240613145258_tmnt TCTCCTGCGATTACAGCTTGATTGGCTGTGGG(+AGGACA)GATCCACCGAGAGAGCTGCCCTCTACCCACA NGG 10 8 17 8 24 0 100 5 10
    240613145257_q0lu AACAAAACAAAACAAAAAAACCTGATTTGATG(+AGGACA)GTACCTCAAAGATACACTAACTACCAATTTT NGG 10 8 17 8 24 0 100 5 10
    240613145256_iplq TGGAGAAAATTGGTAGTTAGTGTATCTTTGAG(+AGGACA)GTACCATCAAATCAGGTTTTTTTGTTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240613145253_vio6 TCTTCCAAAAGAGCAACACTACCTCCCCGAGG(+AGGACA)GTACCCTATCCTCAGAGTCAGGGTGGAGCTC NGG 10 8 17 8 24 0 100 5 10