Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613145205_c6r8 CGCCTCCCTGGTGATGCCACCTAGGAGGGTTG(+AGGACA)GATCCTGGACTCAGGCCCACCTTCTCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240613145204_987f ATAATGGCCCATCTTTTGGGATACGGTCCTTG(+AGGACA)GATCCACTCTTATTTCATCCCCAGTCCTTGT NGG 10 8 17 8 24 0 100 5 10
    240613145202_gz2w CCTGCACAAGGACTGGGGATGAAATAAGAGTG(+AGGACA)GATCCAAGGACCGTATCCCAAAAGATGGGCC NGG 10 8 17 8 24 0 100 5 10
    240613145200_8tfn CAGCCACTCAGCCCTTCCCTCCTCCAGCCCTG(+AGGACA)GTACCCCCGCGTTCCAAAGCCGCCCAGTTCT NGG 10 8 17 8 24 0 100 5 10
    240613145157_akp9 CTCCCAGAACTGGGCGGCTTTGGAACGCGGGG(+AGGACA)GTACCAGGGCTGGAGGAGGGAAGGGCTGAGT NGG 10 8 17 8 24 0 100 5 10
    240613145156_10sg AGCTGGGCCGGGGCCCAAGGTGCCCGAGAGAG(+AGGACA)GTACCCTCGGGAGGACTGGGGTGGGGGTGGT NGG 10 8 17 8 24 0 100 5 10
    240613145154_73d2 GGAGCACCACCCCCACCCCAGTCCTCCCGAGG(+AGGACA)GTACCTCTCTCGGGCACCTTGGGCCCCGGCC NGG 10 8 17 8 24 0 100 5 10
    240613145152_8ytl GGGCCCAGTCCCAGTGCCCCAGGACTCCTGTG(+AGGACA)GATCCAACCAGCTTCCTCTCCCCCGGGACTA NGG 10 8 17 8 24 0 100 5 10
    240613145150_cd2a AACTTTAGTCCCGGGGGAGAGGAAGCTGGTTG(+AGGACA)GATCCACAGGAGTCCTGGGGCACTGGGACTG NGG 10 8 17 8 24 0 100 5 10
    240613145149_jujk AAACCCCTGGTGCTCTCCCAGACCAGAAAAGG(+AGGACA)GTACCAGATCTCCTGGAACTGGAATTACAAG NGG 10 8 17 8 24 0 100 5 10