Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613150032_3o31 CTCCCTTGTATCCTGTACAAGACGCCAGCGCG(+AGGACA)GATCCCACGCTTCGTAAGAAAGGAGTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240613150030_iuli CAAGGAAAGCACTCCTTTCTTACGAAGCGTGG(+AGGACA)GATCCGCGCTGGCGTCTTGTACAGGATACAA NGG 10 8 17 8 24 0 100 5 10
    240613150029_r38t TATAGCTAAGTTTACACCAACCAAATTATTTG(+AGGACA)GTACCAATTCTTGTAAATACTGGGTATGCTG NGG 10 8 17 8 24 0 100 5 10
    240613150028_hw8l TACCCCAGCATACCCAGTATTTACAAGAATTG(+AGGACA)GTACCAAATAATTTGGTTGGTGTAAACTTAG NGG 10 8 17 8 24 0 100 5 10
    240613150027_l1n7 CTCCCTTGTATCCTGTACAAGACGCCAGCGCG(+AGGACA)GATCCCACGCTTCGTAAGAAAGGAGTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240613150025_f98z CAAGGAAAGCACTCCTTTCTTACGAAGCGTGG(+AGGACA)GATCCGCGCTGGCGTCTTGTACAGGATACAA NGG 10 8 17 8 24 0 100 5 10
    240613150024_60qe TCAGGTTGTCACAGGAATACCCCACTCTGCTG(+AGGACA)GTACCAATTTGTCTTAGTCAGGGTTTCTATT NGG 10 8 17 8 24 0 100 5 10
    240613150023_mtwd GCAGGAATAGAAACCCTGACTAAGACAAATTG(+AGGACA)GTACCAGCAGAGTGGGGTATTCCTGTGACAA NGG 10 8 17 8 24 0 100 5 10
    240613150021_mpc4 CAAAAGTCAATCTCCAACCGTCCACTGTCTGG(+AGGACA)GATCCAACTCACGATCTTCTGGGCTCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240613150020_6kel GCCCTTGGAGGAGCCCAGAAGATCGTGAGTTG(+AGGACA)GATCCCAGACAGTGGACGGTTGGAGATTGAC NGG 10 8 17 8 24 0 100 5 10