Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613150328_dje3 GTGGTCCCTAGGCAAAAGGAGGGGGATTCTGG(+AGGACA)GATCCTAAAAGGCTTTATTTTTTGACTTAAA NGG 10 8 17 8 24 0 100 5 10
    240613150327_83co ACCCCTATGAAAGGGTAGATTGACCTCCAAAG(+AGGACA)GTACCGCTACCCACAGGCTGAGAACGACTAC NGG 10 8 17 8 24 0 100 5 10
    240613150325_zwur CTAGAGTAGTCGTTCTCAGCCTGTGGGTAGCG(+AGGACA)GTACCTTTGGAGGTCAATCTACCCTTTCATA NGG 10 8 17 8 24 0 100 5 10
    240613150324_35yc TGGGTTATGGACCAGTAAGACTTTGGGTTAGG(+AGGACA)GATCCCCTAGTCTCTTCTGCCTATTGTGTGC NGG 10 8 17 8 24 0 100 5 10
    240613150322_szw2 TCAGTGCACACAATAGGCAGAAGAGACTAGGG(+AGGACA)GATCCCTAACCCAAAGTCTTACTGGTCCATA NGG 10 8 17 8 24 0 100 5 10
    240613150320_n2o2 CTGAGCTGAGGGTTGGCTATGGTCTCTCATGG(+AGGACA)GTACCCTGGGGATGCCAGCTACTCATGTGCA NGG 10 8 17 8 24 0 100 5 10
    240613150317_9jdk GTGAGTGCACATGAGTAGCTGGCATCCCCAGG(+AGGACA)GTACCCATGAGAGACCATAGCCAACCCTCAG NGG 10 8 17 8 24 0 100 5 10
    240613150315_0fea GCTATATTATTCTTGCCAGAATCTTGTGGAGG(+AGGACA)GATCCCCACTCATCCCCTGACTTCTATTCTT NGG 10 8 17 8 24 0 100 5 10
    240613150313_b6a6 GTACAAAGAATAGAAGTCAGGGGATGAGTGGG(+AGGACA)GATCCCTCCACAAGATTCTGGCAAGAATAAT NGG 10 8 17 8 24 0 100 5 10
    240613150312_8yj0 GGAAATTTGGAAATTGCCTTGCAGATATTTCG(+AGGACA)GTACCGCTGAGTTTTCTTTCTTTTCCCAAGT NGG 10 8 17 8 24 0 100 5 10