Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613150149_mnpf GCAGGAAGTCCTGAGGTTAGGGACTGGCTATG(+AGGACA)GTACCCAGGACCCGGTCAGTAGTACCTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613150147_f7dg TGAGACAGCAGGTACTACTGACCGGGTCCTGG(+AGGACA)GTACCATAGCCAGTCCCTAACCTCAGGACTT NGG 10 8 17 8 24 0 100 5 10
    240613150146_6m49 GAGAGTGTCTTTTCTGCCTAAGCTTGGCAATG(+AGGACA)GATCCCGGAAAGAGACGGCTCTCCCGGCTGC NGG 10 8 17 8 24 0 100 5 10
    240613150144_tpvp CATGAGCAGCCGGGAGAGCCGTCTCTTTCCGG(+AGGACA)GATCCATTGCCAAGCTTAGGCAGAAAAGACA NGG 10 8 17 8 24 0 100 5 10
    240613150142_xcw9 CTGGCCCTGTGCCTGTGGCACTTTCGCCTATG(+AGGACA)GTACCATCTCCCCAGTGCAAGTCATGGACTG NGG 10 8 17 8 24 0 100 5 10
    240613150141_2fqh GGAGGCAGTCCATGACTTGCACTGGGGAGATG(+AGGACA)GTACCATAGGCGAAAGTGCCACAGGCACAGG NGG 10 8 17 8 24 0 100 5 10
    240613150139_l3p3 TGGAACTCACACCAGGCATAAACAGACAGAGG(+AGGACA)GATCCGGAACGGCAAGACCTCGGAACACTAC NGG 10 8 17 8 24 0 100 5 10
    240613150137_83qh TCTGAGTAGTGTTCCGAGGTCTTGCCGTTCCG(+AGGACA)GATCCCTCTGTCTGTTTATGCCTGGTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240613150135_fpf1 GGAGGGACTCTCCTGGATTCAGACCTCCACAG(+AGGACA)GTACCCTGTGACTACCAAAGTTCAAGGGGAA NGG 10 8 17 8 24 0 100 5 10
    240613150134_vo1y AGCCATTCCCCTTGAACTTTGGTAGTCACAGG(+AGGACA)GTACCTGTGGAGGTCTGAATCCAGGAGAGTC NGG 10 8 17 8 24 0 100 5 10