Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240731021549_f96u CATTATTACGTGTCCCGGGACGCTCTGCTCGGTGGGCCTGGGAATGTGTGGGCTGATATTGATTTTGCGCATGAGTCGGCGGCGTTTCTGCCTTGGCATCGGGTGTATCTGCTGTTTTGGGAGCATGAGATCCGGAAGCTGACGGGAGACTTTAACTTCACCATCCCGTACTGGGACTGGCGGGACGCGCAGGACTGTCAGGTGTGCACGGATGAGCTGATGGGGGCGCGCAGCAGCCTCAACCGCAGCCTGATCAGCCCCTCCTCTGTGTTCTCATCCTGG(/A)AAGGTAAAGCAGACTTTTATTTGACTTTTTTTAAAACAAGAATGTTAGTTCTAGGGGCGGTATGGTGGCTCAGTGGTTATCCCTATCGCTTCACAGCAAGAAGGTCACTGGTTCAAGTCCAAGTTAAATTGGTTTGAGTTGAATTGGCTGTAGTGTATGA NGG 10 8 17 8 24 0 100 5 10
    240731021534_i57q CATTATTACGTGTCCCGGGACGCTCTGCTCGGTGGGCCTGGGAATGTGTGGGCTGATATTGATTTTGCGCATGAGTCGGCGGCGTTTCTGCCTTGGCATCGGGTGTATCTGCTGTTTTGGGAGCATGAGATCCGGAAGCTGACGGGAGACTTTAACTTCACCATCCCGTACTGGGACTGGCGGGACGCGCAGGACTGTCAGGTGTGCACGGATGAGCTGATGGGGGCGCGCAGCAGCCTCAACCGCAGCCTGATCAGCCCCTCCTCTGTGTTCTCATCCTGG(/A)AAGGTAAAGCAGACTTTTATTTGACTTTTTTTAAAACAAGAATGTTAGTTCTAGGGGCGGTATGGTGGCTCAGTGGTTATCCCTATCGCTTCACAGCAAGAAGGTCACTGGTTCAAGTCCAAGTTAAATTGGTTTGAGTTGAATTGGCTGTAGTGTATGAGTGTTTGTATGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTGGGAGTGTAGAGGGTA NGG 10 8 17 8 24 0 100 5 10
    240731021254_bg01 CTTCTTACTGAGGACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTCCGAGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCCTACAGCCCGTCATCGGCTCGGCGAGCGACTACTGTAGGTCGTCATG(/A)GGGCCGAAGGAGACCGTACATACTCTTACTGGGGATTCTGATGTTAGTGGGCATGACTTTATTTCTAAATGGAGATGCAGTCACAACAGGTGGGTGAAGGTCAAGTAAACTTGATAGATTTTACTTCATTTTGAAGACAAAGCACTAAATACACAAAGGAAGTAGATGAGTTTCAGGGTCCACACAGGTCATGGTATTTCTGGAATATCAAGGGTTTTAAAAGCTCTTTTCCAAACAGTGAAAGTCAGATTTTTTTATTTAATTATTTTCGGTCCAAGTCATGGAATATCAGGGATTTCTGTTTTTCACTTACAGTTGAAGTCAGAATTATTAGCCCCCCAGTTTATTTTTCCCCTAATTTCTG NGG 10 8 17 8 24 0 100 5 10
    240731021011_cf68 CTTCTTACTGAGGACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTCCGAGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCCTACAGCCCGTCATCGGCTCGGCGAGCGACTACTGTAGGTCGTCATGG(/A)GGCCGAAGGAGACCGTACATACTCTTACTGGGGATTCTGATGTTAGTGGGCATGACTTTATTTCTAAATGGAGATGCAGTCACAACAGGTGGGTGAAGGTCAAGTAAACTTGATAGATTTTACTTCATTTTGAAGACAAAGCACTAAATACACAAAGGAAGTAGATGAGTTTCAGGGTCCACACAGGTCATGGTATTTCTGGAATATCAAGGGTTTTAAAAGCTCTTTTCCAAACAGTGAAAGTCAGATTTTTTTATTTAATTATTTTCGGTCCAAGTCATGGAATATCAGGGATTTCTGTTTTTCACTTACAGTTGAAGTCAGAATTATTAGCCCCCCAGTTTATTTTTCCCCTAATTTCTG NGG 10 8 17 8 24 0 100 5 10
    240731020934_tzrq CTTCTTACTGAGGACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTCCGAGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCCTACAGCCCGTCATCGGCTCGGCGAGCGACTACTGTAGGTCGTCATGG(/A)GGCCGAAGGAGACCGTACATACTCTTACTGGGGATTCTGATGTTAGTGGGCATGACTTTATTTCTAAATGGAGATGCAGTCACAACAGGTGGGTGAAGGTCAAGTAAACTTGATAGATTTTACTTCATTTTGAAGACAAAGCACTAAATACACAAAGGAAGTAGATGAGTTTCAGGGTCCACACAGGTCATGGTATTTCTGGAATATCAAGGGTTTTAAAAGCTCTTTTCCAAACAGTGAAAGTCAGATTTTTTTATTTAATTATTTTCGGTCCAAGTCATGGAATATCAGGGATTTCTGTTTTTCACTTACAGTTGAAGTCAGAATTATTAGCCCCCCAGTTTATTTTTCCCCTAATTTCTG NGG 10 8 17 8 24 0 100 5 10
    240730164642_0bxb CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240730163345_6l7y cgagcagaaggaaaatgtggaaaaagaggggctgtgtaatgcatgtttgtggagggaaaa(/g)cactacatcctcaagactagaagccataaaaatccaaatcctcagtaaacttcgcctggaaacagctcctaacatcagcaaagatgctatcagacaacttttgcccaaggctcctccactcctggaactgattgatcagttcgatgtccagagagatgccagcagtgacggctccttggaagacgatgactaccacgccaggacggaaacggtcattaccatgcccacggagtgtgagtagtcctgctggtgcagagcaacgactctgctgactgctgttctagtgttcatgagaaaccgatctattttcaggctcttttaacaagctgctggcttgtatgtaaggaggaggggaaagagcttttttcaagatttcatga NGG 10 8 17 8 24 0 100 5 10
    240730163217_uu5b cgagcagaaggaaaatgtggaaaaagaggggctgtgtaatgcatgtttgtggagggaa(/c)aacactacatcctcaagactagaagccataaaaatccaaatcctcagtaaacttcgcctggaaacagctcctaacatcagcaaagatgctatcagacaacttttgcccaaggctcctccactcctggaactgattgatcagttcgatgtccagagagatgccagcagtgacggctccttggaagacgatgactaccacgccaggacggaaacggtcattaccatgcccacggagtgtgagtagtcctgctggtgcagagcaacgactctgctgactgctgttctagtgttcatgagaaaccgatctattttcaggctcttttaacaagctgctggcttgtatgtaaggaggaggggaaagagcttttttcaagatttcatga NGG 10 8 17 8 24 0 100 5 10
    240730161741_lye8 cgagcagaaggaaaatgtggaaaaagaggggctgtgtaatgcatgtttgtggagggaaaacactacatcctcaagactagaagccataaaaatccaaatcctcagtaaacttcgcctggaaacagctcctaacatcagcaaagatgctatcagacaacttttgcccaaggctcctccactcctggaactgattgatcagttcgatgtccaga(/c)gagatgccagcagtgacggctccttggaagacgatgactaccacgccaggacggaaacggtcattaccatgcccacggagtgtgagtagtcctgctggtgcagagcaacgactctgctgactgctgttctagtgttcatgagaaaccgatctattttcaggctcttttaacaagctgctggcttgtatgtaaggaggaggggaaagagcttttttcaagatttcatga NGG 10 8 17 8 24 0 100 5 10
    240730161426_wqrf gtttgtggagggaaaacactacatcctcaagactagaagccataaaaatccaaatcctcagtaaacttcgcctggaaacagctcctaacatcagcaaagatgctatcagacaacttttgcccaaggctcctccactcctggaactgattgatcagttcgatgtccagagagatgccagcagtgacggctccttggaagacgatgactacca(/c)cgccaggacggaaacggtcattaccatgcccacggagtgtgagtagtcctgctggtgca NGG 10 8 17 8 24 0 100 5 10