Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240731151658_r501 CTTCTTACTGAGGACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTCCGAGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCCTACAGCCCGTCATCGGCTCGGCGAGCGACTACTGTAGGTCG(+TGA)TCATGGGGCCGAAGGAGACCGTACATACTCTTACTGGGGATTCTGATGTTAGTGGGCATGACTTTATTTCTAAATGGAGATGCAGTCACAACAGGTGGGTGAAGGTCAAGTAAACTTGATAGATTTTACTTCATTTTGAAGACAAAGCACTAAATACACAAAGGAAGTAGATGAGTTTCAGGGTCCACACAGGTCATGGTATTTCTGGAATATCAAGGGTTTTAAAAGCTCTTTTCCAAACAGTGAAAGTCAGATTTTTTTATTTAATTATTTTCGGTCCAAGTCATGGAATATCAGGGATTTCTGTTTTTCACTTACAGTTGAAGTCAGAATTATTAGCCCCCCAGTTTATTTTTCCCCTAATTTCTG NGG 10 8 17 8 24 0 100 5 10
    240731151649_23i9 CTTCTTACTGAGGACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTCCGAGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCCTACAGCCCGTCATCGGCTCGGCGAGCGACTACTGTAGGTCGTCA(+TGA)TGGGGCCGAAGGAGACCGTACATACTCTTACTGGGGATTCTGATGTTAGTGGGCATGACTTTATTTCTAAATGGAGATGCAGTCACAACAGGTGGGTGAAGGTCAAGTAAACTTGATAGATTTTACTTCATTTTGAAGACAAAGCACTAAATACACAAAGGAAGTAGATGAGTTTCAGGGTCCACACAGGTCATGGTATTTCTGGAATATCAAGGGTTTTAAAAGCTCTTTTCCAAACAGTGAAAGTCAGATTTTTTTATTTAATTATTTTCGGTCCAAGTCATGGAATATCAGGGATTTCTGTTTTTCACTTACAGTTGAAGTCAGAATTATTAGCCCCCCAGTTTATTTTTCCCCTAATTTCTG NGG 10 8 17 8 24 0 100 5 10
    240731151625_ort1 AGTGGTGGAGCCTCCGAGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCCTACAGCCCGTCATCGGCTCGGCGAGCGACTACTGTAGGTCGTCATGGGGCCGAAGG(+TGA)AGACCGTACATACTCTTACTGGGGATTCTGATGTTAGTGGGCATGACTTTATTTCTAAATGGAGATGCAGTCACAACAGGTGGGTGAAGGTCAAGTAAACTTGATAGATTTTACTTCATTTTGAAGACAAAGCACTAAATACACAAAGGAAGTAGATGAGTTTCAGGGTCCACACAGGTCATGGTATTTCTGGAATATCAAGGGTTTTAAAAGCTCTTTTCCAAACAGTGAAAGTCAGATTTTTTTATTTAATTATTTTCGGTCCAAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240731151607_up9q CCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCCTACAGCCCGTCATCGGCTCGGCGAGCGACTACTGTAGGTCGTCATGGGGCCGA(+TGA)AGGAGACCGTACATACTCTTACTGGGGATTCTGATGTTAGTGGGCATGACTTTATTTCTAAATGGAGATGCAGTCACAA NGG 10 8 17 8 24 0 100 5 10
    240731151556_rbz2 CCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCCTACAGCCCGTCATCGGCTCGGCGAGCGACTACTGTAGGTCGTCATGGGGC(+TGA)CGAAGGAGACCGTACATACTCTTACTGGGGATTCTGATGTTAGTGGGCATGACTTTATTTCTAAATGGAGATGCAGTCACAA NGG 10 8 17 8 24 0 100 5 10
    240731151533_voeb CTTCTTACTGAGGACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTCCGAGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCCTACAGCCCGTCATCGGCTCGGCGAGCGACTACTGTAGGTCGTCATGG(+TGA)GGCCGAAGGAGACCGTACATACTCTTACTGGGGATTCTGATGTTAGTGGGCATGACTTTATTTCTAAATGGAGATGCAGTCACAACAGGTGGGTGAAGGTCAAGTAAACTTGATAGATTTTACTTCATTTTGAAGACAAAGCACTAAATACACAAAGGAAGTAGATGAGTTTCAGGGTCCACACAGGTCATGGTATTTCTGGAATATCAAGGGTTTTAAAAGCTCTTTTCCAAACAGTGAAAGTCAGATTTTTTTATTTAATTATTTTCGGTCCAAGTCATGGAATATCAGGGATTTCTGTTTTTCACTTACAGTTGAAGTCAGAATTATTAGCCCCCCAGTTTATTTTTCCCCTAATTTCTG NGG 10 8 17 8 24 0 100 5 10
    240731151522_f8y4 CTTCTTACTGAGGACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTCCGAGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCCTACAGCCCGTCATCGGCTCGGCGAGCGACTACTGTAGGTCGTCATGGGGC(+TGA)CGAAGGAGACCGTACATACTCTTACTGGGGATTCTGATGTTAGTGGGCATGACTTTATTTCTAAATGGAGATGCAGTCACAACAGGTGGGTGAAGGTCAAGTAAACTTGATAGATTTTACTTCATTTTGAAGACAAAGCACTAAATACACAAAGGAAGTAGATGAGTTTCAGGGTCCACACAGGTCATGGTATTTCTGGAATATCAAGGGTTTTAAAAGCTCTTTTCCAAACAGTGAAAGTCAGATTTTTTTATTTAATTATTTTCGGTCCAAGTCATGGAATATCAGGGATTTCTGTTTTTCACTTACAGTTGAAGTCAGAATTATTAGCCCCCCAGTTTATTTTTCCCCTAATTTCTG NGG 10 8 17 8 24 0 100 5 10
    240731151423_txew CTTCTTACTGAGGACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTCCGAGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCCTACAGCCCGTCATCGGCTCGGCGAGCGACTACTGTAGGTCGTCATGGGGC(+TGA)CGAAGGAGACCGTACATACTCTTACTGGGGATTCTGATGTTAGTGGGCATGACTTTATTTCTAAATGGAGATGCAGTCACAACAGGTGGGTGAAGGTCAAGTAAACTTGATAGATTTTACTTCATTTTGAAGACAAAGCACTAAATACACAAAGGAAGTAGATGAGTTTCAGGGTCCACACAGGTCATGGTATTTCTGGAATATCAAGGGTTTTAAAAGCTCTTTTCCAAACAGTGAAAGTCAGATTTTTTTATTTAATTATTTTCGGTCCAAGTCATGGAATATCAGGGATTTCTGTTTTTCACTTACAGTTGAAGTCAGAATTATTAGCCCCCCAGTTTATTTTTCCCCTAATTTCTG NGG 10 8 17 8 24 0 100 5 10
    240731151119_plbz CTTCTTACTGAGGACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTCCGAGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCCTACAGCCCGTCATCGGCTCGGCGAGCGACTACTGTAGGTCGTCATGG(+TGA)GGCCGAAGGAGACCGTACATACTCTTACTGGGGATTCTGATGTTAGTGGGCATGACTTTATTTCTAAATGGAGATGCAGTCACAACAGGTGGGTGAAGGTCAAGTAAACTTGATAGATTTTACTTCATTTTGAAGACAAAGCACTAAATACACAAAGGAAGTAGATGAGTTTCAGGGTCCACACAGGTCATGGTATTTCTGGAATATCAAGGGTTTTAAAAGCTCTTTTCCAAACAGTGAAAGTCAGATTTTTTTATTTAATTATTTTCGGTCCAAGTCATGGAATATCAGGGATTTCTGTTTTTCACTTACAGTTGAAGTCAGAATTATTAGCCCCCCAGTTTATTTTTCCCCTAATTTCTG NGG 10 8 17 8 24 0 100 5 10
    240731151108_x9j7 CTTCTTACTGAGGACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTCCGAGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCCTACAGCCCGTCATCGGCTCGGCGAGCGACTACTGTAGGTCGTCATGG(+TGA)GGCCGAAGGAGACCGTACATACTCTTACTGGGGATTCTGATGTTAGTGGGCATGACTTTATTTCTAAATGGAGATGCAGTCACAACAGGTGGGTGAAGGTCAAGTAAACTTGATAGATTTTACTTCATTTTGAAGACAAAGCACTAAATACACAAAGGAAGTAGATGAGTTTCAGGGTCCACACAGGTCATGGTATTTCTGGAATATCAAGGGTTTTAAAAGCTCTTTTCCAAACAGTGAAAGTCAGATTTTTTTATTTAATTATTTTCGGTCCAAGTCATGGAATATCAGGGATTTCTGTTTTTCACTTACAGTTGAAGTCAGAATTATTAGCCCCCCAGTTTATTTTTCCCCTAATTTCTG NGG 10 8 17 8 24 0 100 5 10