Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240805114305_t8rp ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCG(-ATG)GGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTCACGGCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAAGCAACGGAGCGCGGTGAAGCCCATTTTTCTCCTTCCTCGCAGCCGCGCCGGGCAGTTCGCGGCGCGCGGCCCCCACGCTCCCGCCCGAGATGAAT NGG 10 8 17 8 24 0 100 5 10
    240805113729_s2c2 ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCGATGGGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTC(-ACG)GCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAAGCAACGGAGCGCGGTGAAGCCCATTTTTCTCCTTCCTCGCAGCCGCGCCGGGCAGTTCGCGGCGCGCGGCCCCCACGCTCCCGCCCGAGATGAATGCTGCGGCAGAAGCCGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240805112139_qlnb ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCGATGGGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTCACG(-GCT)CCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAAGCAACGGAGCGCGGTGAAGCCCATTTTTCTCCTTCCTCGCAGCCGCGCCGGGCAGTTCGCGGCGCGCGGCCCCCACGCTCCCGCCCGAGATGAATGCTGCGGCAGAAGCCGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240805112122_pdcn ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCGATGGGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTC(-ACG)GCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAAGCAACGGAGCGCGGTGAAGCCCATTTTTCTCCTTCCTCGCAGCCGCGCCGGGCAGTTCGCGGCGCGCGGCCCCCACGCTCCCGCCCGAGATGAATGCTGCGGCAGAAGCCGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240804184603_kqqm ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCGATGGGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTC(-ACG)GCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAAGCAACGGAGCGCGGTGAAGCCCATTTTTCTCCTTCCTCGCAGCCGCGCCGGGCAGTTCGCGGCGCGCGGCCCCCACGCTCCCGCCCGAGATGAATGCTGCGGCAGAAGCCGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240804183759_j32h ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCGATGGGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTC(-ACG)GCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAAGCAACGGAGCGCGGTGAAGCCCATTTTTCTCCTTCCTCGCAGCCGCGCCGGGCAGTTCGCGGCGCGCGGCCCCCACGCTCCCGCCCGAGATGAATGCTGCGGCAGAAGCCGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240804182917_4kci ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCGATGGGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTC(-ACG)GCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAAGCAACGGAGCGCGGTGAAGCCCATTTTTCTCCTTCCTCGCAGCCGCGCCGGGCAGTTCGCGGCGCGCGGCCCCCACGCTCCCGCCCGAGATGAATGCTGCGGCAGAAGCCGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240804181124_izk3 ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCG(-ATG)GGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTCACGGCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAAGCAACGGAGCGCGGTGAAGCCCATTTTTCTCCTTCCTCGCAGCCGCGCCGGGCAGTTCGCGGCGCGCGGCCCCCACGCTCCCGCCCGAGATGAAT NGG 10 8 17 8 24 0 100 5 10
    240803155238_x043 ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCG(-ATG)GGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTCACGGCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAAGCAACGGAGCGCGGTGAAGCCCATTTTTCTCCTTCCTCGCAGCCGCGCCGGGCAGTTCGCGGCGCGCGGCCCCCACGCTCCCGCCCGAGATGAAT NGG 10 8 17 8 24 0 100 5 10
    240803153201_y7uu ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCG(-ATG)GGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTCACGGCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAAGCAACGGAGCGCGGTGAAGCCCATTTTTCTCCTTCCTCGCAGCCGCGCCGGGCAGTTCGCGGCGCGCGGCCCCCACGCTCCCGCCCGAGATGAAT NGG 10 8 17 8 24 0 100 5 10