Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240811134624_fmke tcccacctgcgcaagctgcgtaagcggctcctccgcgatgccgatgacctgcagaagc(/t)gcctggcagtgtaccaggccggggcccgcgagggcgccgagcgcggcctcagcgccatccgcgagcgcctggggcccctggtggaacag NGG 10 8 17 8 24 0 100 5 10
    240810233211_rs15 AGAAGCTTCTACCGTCCCTTTCATTTGGGCAGTGAACAAGGACCAATCCACGTG(+A)GTTACCGGAGAGTTTGTTCGATGAGAAAAAATGTCTGATTATTAAAGGGTGGGCACCGCAACTAAC NGG 10 8 17 8 24 0 100 5 10
    240810220206_j5og AAACACAAGCACAAATCGGTCCTCTACGTCTCTTTTGGGAGCACAGTTAGATTCCCAGAGGAGCAACTCGCTGAAATCGCAAAAGCTCTAGAAGCTTCTACCGTCCCTT(+AG)TCATTTGGGCAGTGAACAAGGACCAATCCACGTGGTTACCGGAGAGTTTGTTCGATGAGAAAAAATGTCTGATTATTAAAGGGTGGGCACCGCAACTAACCATCTAATAGATC NGG 10 8 17 8 24 0 100 5 10
    240810220156_8mgz AAACACAAGCACAAATCGGTCCTCTACGTCTCTTTTGGGAGCACAGTTAGATTCCCAGAGGAGCAACTCGCTGAAATCGCAAAAGCTCTAGAAGCTTCTACCGTCCCTT(+AG)TCATTTGGGCAGTGAACAAGGACCAATCCACGTGGTTACCGGAGAGTTTGTTCGATGAGAAAAAATGTCTGATTATTAAAGGGTGGGCACCGCAACTAACCATCTAATAGATC NGG 10 8 17 8 24 0 100 5 10
    240810220151_jz25 AAACACAAGCACAAATCGGTCCTCTACGTCTCTTTTGGGAGCACAGTTAGATTCCCAGAGGAGCAACTCGCTGAAATCGCAAAAGCTCTAGAAGCTTCTACCGTCCCTT(+AG)TCATTTGGGCAGTGAACAAGGACCAATCCACGTGGTTACCGGAGAGTTTGTTCGATGAGAAAAAATGTCTGATTATTAAAGGGTGGGCACCGCAACTAACCATCTAATAGATC NGG 10 8 17 8 24 0 100 5 10
    240810142824_ga85 ccggctgggcgcggacatggaggacgtgtgcggccgcctggtgcagtaccgcggcgaggtgcaggccatgctcggccagagcaccgaggagctgcgggtgcgcctcgcctcccacctgcgcaagctgcgtaagcggctcctccgcgatgccgatgacctgcagaagc(/t)gcctggcagtgtaccaggccggggcccgcgagggcgccgagcgcggcctcagcgccatccgcgagcgcctggggcccctggtggaacagggccgcgtgcgggccgccactgtgggctccctggccggccagccgctacaggagcgggcccaggcctggggcgagcggctgcgcgcgcggatggaggagatggg NGG 10 8 17 8 24 0 100 5 10
    240810142531_06jh ccggctgggcgcggacatggaggacgtgtgcggccgcctggtgcagtaccgcggcgaggtgcaggccatgctcggccagagcaccgaggagctgcgggtgcgcctcgcctcccacctgcgcaagctgcgtaagcggctcctccgcgatgccgatgacctgcagaagc(/t)gcctggcagtgtaccaggccggggcccgcgagggcgccgagcgcggcctcagcgccatccgcgagcgcctggggcccctggtggaacagggccgcgtgcgggccgccactgtgggctccctggccggccagccgctacaggagcgggcccaggcctggggcgagcggctgcgcgcgcggatggaggagatggg NGG 10 8 17 8 24 0 100 5 10
    240810110825_9civ CGATCTGGTAAGGCCTGCGGATGAGAGGAATGCTTTTGATGAATTGCTCGATCGAACCAGAGAATCTGAGGATCTAAGCTACGGTATCGTTCATGATACTTTTTACGAGCTAGAACCTGCCTACGCTGACTACTATCAAAAGATGAAGAAAACCAAAT(+AA)GTTGGCAAATTGGTCCCATTTCCTATTTTTCTTCCAAATTA NGG 10 8 17 8 24 0 100 5 10
    240810105654_l15r AAAAGCTAGAGGAGTAAAAGGGTGGCATATAGGTCCACTTGCTCTGTTTATCAACAAATATGAAGCGGAAATTAGTTCTAAACAAATTTCCAATTCGAATATTAATTCATGTTCTGACCCTTG(-G)AAAGGGTACGGTGATTGTTTCAATTGGCTTGAAAATCAACAACCTAACTCCGTTCTCTTTGTTTGCTTTGGAAGCATGATAAGATTTTCCGATGATCAGCTTAAGGAAATGGC NGG 10 8 17 8 24 0 100 5 10
    240810105607_menz AAAAGCTAGAGGAGTAAAAGGGTGGCATATAGGTCCACTTGCTCTGTTTATCAACAAATATGAAGCGGAAATTAGTTCTAAACAAATTTCCAATTCGAATATTAATTCATGTTCTGACCCTTG(-G)AAAGGGTACGGTGATTGTTTCAATTGGCTTGAAAATCAACAACCTAACTCCGTTCTCTTTGTTTGCTTTGGAAGCATGATAAGATTTTCCGATGATCAGCTTAAGGAAATGGC NGG 10 8 17 8 24 0 100 5 10