Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240816053705_ccbm CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 30 45 0 100 5 10
    240816043148_r6mz CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240815221957_ecp0 ggagaagggacagggctggtggagcccaggcctcttgtctctgggatccctctctcacgggccctcctggtctctaggccctcggacctccgcg(+TAAA)ctcctggctttcgccctgctctgcctgccctggactcgggaggtgggcgccttcccagccatgcccttgtccagcctatttgccaacgccgtgctccgggccc NGG 10 8 17 8 24 0 100 5 10
    240815221930_ykd3 ggagaagggacagggctggtggagcccaggcctcttgtctctgggatccctctctcacgggccctcctggtctctaggccctcggacctccgcg(+TAA)ctcctggctttcgccctgctctgcctgccctggactcgggaggtgggcgccttcccagccatgcccttgtccagcctatttgccaacgccgtgctccgggccc NGG 10 8 17 8 24 0 100 5 10
    240815221455_tlzr ggagaagggacagggctggtggagcccaggcctcttgtctctgggatccctctctcacgggccctcctggtctctaggccctcggacctccgcg(+TAAA)ctcctggctttcgccctgctctgcctgccctggactcgggaggtgggcgccttcccagccatgcccttgtccagcctatttgccaacgccgtgctccgggccc NGG 10 8 17 8 24 0 100 5 10
    240814141212_5j2n GGAGCGCCACGAGACGCTGGTCCAGTGGCGCGGCCGCCTCGGCGGGTCCGGGTTCGAGCCCGTGCACCTGGGATCCAACGCCTACAAGCAGGCAAGCACGCTGCTGGCCCTCTTCGCCGGCGGCGACGGGTACAGGGTGGAGGAGAAGGACGGGTGCCTGACTCTGGGATGGCATACGCGCCCGC(/T)TCATCGCCACCTCGGCGTGGCGCGTCGCCGCTCCGTGACGTGCCCACCGTCCGGCACAGCACAGCACAGCACAGCACAGTGGAAGCTAAGACGTCAGTGAACGCTGCTGAACTGCAG NGG 10 8 17 8 24 0 100 5 10
    240814141004_ep8x GGAGCGCCACGAGACGCTGGTCCAGTGGCGCGGCCGCCTCGGCGGGTCCGGGTTCGAGCCCGTGCACCTGGGATCCAACGCCTACAAGCAGGCAAGCACGCTGCTGGCCCTCTTCGCCGGCGGCGACGGGTACAGGG(/A)TGGAGGAGAAGGACGGGTGCCTGACTCTGGGATGGCATACGCGCCCGCTCATCGCCACCTCGGCGTGGCGCGTCGCCGCTCCGTGACGTGCCCACCGTCCGGCACAGCACAGCACAGCACAGCACAGTGGAAGCTAAGACGTCAGTGAACGCTGCTGAACTGCAG NGG 10 8 17 8 24 0 100 5 10
    240814140702_3e8n GGAGCGCCACGAGACGCTGGTCCAGTGGCGCGGCCGCCTCGGCGGGTCCGGGTTCGAGCCCGTGCACCTGGGATCCAACGCCTACAAGCAGGCAAGCACGCTGCTGGCCCTCTTCGCCGGCGGCGACG(/A)GGTACAGGGTGGAGGAGAAGGACGGGTGCCTGACTCTGGGATGGCATACGCGCCCGCTCATCGCCACCTCGGCGTGGCGCGTCGCCGCTCCGTGACGTGCCCACCGTCCGGCACAGCACAGCACAGCACAGCACAGTGGAAGCTAAGACGTCAGTGAACGCTGCTGAACTGCAG NGG 10 8 17 8 24 0 100 5 10
    240814140532_4zd6 GGAGCGCCACGAGACGCTGGTCCAGTGGCGCGGCCGCCTCGGCGGGTCCGGGTTCGAGCCCGTGCACCTGGGATCCAACGCCTACAAGCAGGCAAGCACGCT(/C)GCTGGCCCTCTTCGCCGGCGGCGACGGGTACAGGGTGGAGGAGAAGGACGGGTGCCTGACTCTGGGATGGCATACGCGCCCGCTCATCGCCACCTCGGCGTGGCGCGTCGCCGCTCCGTGACGTGCCCACCGTCCGGCACAGCACAGCACAGCACAGCACAGTGGAAGCTAAGACGTCAGTGAACGCTGCTGAACTGCAG NGG 10 8 17 8 24 0 100 5 10
    240814134809_vstd GGAGCGCCACGAGACGCTGGTCCAGTGGCGCGGCCGCCTCGGCGGGTCCGGGTTCGAGCCCGTGCACCTGGGATCCAACGCCTACAAGCAGGCAAGCACGCT(/C)GCTGGCCCTCTTCGCCGGCGGCGACGGGTACAGGGTGGAGGAGAAGGACGGGTGCCTGACTCTGGGATGGCATACGCGCCCGCTCATCGCCACCTCGGCGTGGCGCGTCGCCGCTCCGTGACGTGCCCACCGTCCGGCACAGCACAGCACAGCACAGCACAGTGGAAGCTAAGACGTCAGTGAACGCTGCTGAACTGCAG NGG 10 8 17 8 24 0 100 5 10