Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240827162609_iuj1 gcatgtatgtggagacaaaacactaaatcttcaagactagaagccataaaaattcaaatcctcagtaaacttcgcctggaaacagctcctaacattagcaaagatgctataagacaacttttgcccaaagctcctccactccgggaactgattgatcagtacgatgtccagagagatgacagcagtgatggctccttggaagatgatgattatcacgctacgacg(+TA)gaaacgatcattaccatgcctacagagtgtaagtagtcctattagtgtatatcaacaattctgctgactgttgttccagtgtttatgagaaacagatctattttcaggctcttttaacaagctgttggcttgtCC NGG 10 8 17 8 24 0 100 5 10
    240827160755_w4zz ttgcccaaagctcctccactccgggaactgattgatcagtacgatgtccagagagatgacagcagtgatggctccttggaagatgatgattatcacgctacgacgTAgaaacgatcattaccatgc(+T)ctacagagtgtaagtagtcctattagtgtatatcaacaattctgctgactgttgttccagtgtttatgagaaacagatctattttcaggctcttttaacaagctgttggcttg NGG 10 8 17 8 24 0 100 5 10
    240827160710_4b44 ataaaaattcaaatcctcagtaaacttcgcctggaaacagctcctaacattagcaaagatgctataagacaacttttgcccaaagctcctccactccgggaactgattgatcagtacgatgtccagagagatgacagcagtgatggctccttggaagatgatgattatcacgctacgacgTAgaaacgatcattaccatgc(+T)ctacagagtgtaagtagtcctattagtgtatatcaacaattctgctgactgttgttccagtgtttatgagaaacagatctattttcaggctcttttaacaagctgttggcttgtacgtaagtaggagggaaaagagtttcttttttcaagatttcatgagaaataaactaatgagactgaaagctgctgtattattgttttccttagctaaacagctgaaaataa NGG 10 8 17 8 24 0 100 5 10
    240827160636_czgw gagagatgacagcagtgatggctccttggaagatgatgattatcacgctacgacg(+TA)gaaacgatcattaccatgcTctacagagtgtaagtagtcctattagtgtatatcaacaattctgctgactgttgttccagtgtttatgagaaacagatctattttcaggctcttttaacaagctgttggcttgtacgtaagtaggagggaaaagagtttcttttttcaagatttcatgagaaataaactaatgagactgaa NGG 10 8 17 8 24 0 100 5 10
    240827160425_jzwq ataaaaattcaaatcctcagtaaacttcgcctggaaacagctcctaacattagcaaagatgctataagacaacttttgcccaaagctcctccactccgggaactgattgatcagtacgatgtccagagagatgacagcagtgatggctccttggaagatgatgattatcacgctacgacgTAgaaacgatcattaccatgc(+T)ctacagagtgtaagtagtcctattagtgtatatcaacaattctgctgactgttgttccagtgtttatgagaaacagatctattttcaggctcttttaacaagctgttggcttgtacgtaagtaggagggaaaagagtttcttttttcaagatttcatgagaaataaactaatgagactgaaagctgctgtattattgttttccttagctaaacagctgaaaataa NGG 10 8 17 8 24 0 100 5 10
    240827160309_4ak9 ataaaaattcaaatcctcagtaaacttcgcctggaaacagctcctaacattagcaaagatgctataagacaacttttgcccaaagctcctccactccgggaactgattgatcagtacgatgtccagagagatgacagcagtgatggctccttggaagatgatgattatcacgctacgacgTAgaaacgatcattaccatgc(+T)ctacagagtgtaagtagtcctattagtgtatatcaacaattctgctgactgttgttccagtgtttatgagaaacagatctattttcaggctcttttaacaagctgttggcttgtacgtaagtaggagggaaaagagtttcttttttcaagatttcatgagaaataaactaatgagactgaaagctgctgtattattgttttccttagctaaacagctgaaaataa NGG 10 8 17 8 24 0 100 5 10
    240827160228_hkl7 ataaaaattcaaatcctcagtaaacttcgcctggaaacagctcctaacattagcaaagatgctataagacaacttttgcccaaagctcctccactccgggaactgattgatcagtacgatgtccagagagatgacagcagtgatggctccttggaagatgatgattatcacgctacgacgTAgaaacgatcattaccatgc(+T)ctacagagtgtaagtagtcctattagtgtatatcaacaattctgctgactgttgttccagtgtttatgagaaacagatctattttcaggctcttttaacaagctgttggcttgtacgtaagtaggagggaaaagagtttcttttttcaagatttcatgagaaataaactaatgagactgaaagctgctgtattattgttttccttagctaaacagctgaaaataa NGG 10 8 17 8 24 0 100 5 10
    240827154634_ba8w aatcttcaagactagaagccataaaaattcaaatcctcagtaaacttcgcctggaaacagctcctaacattagcaaagatgctataagacaacttttgcccaaagctcctccactccgggaactgattgatcagtacgatgtccagagagatgacagcagtgatggctccttggaagatgatgattatcacgctacgacg(+TA)gaaacgatcattaccatgcctacagagtgtaagtagtcctattagtgtatatcaacaattctgctgactgttgttccagtgtttatgagaaacagatctattttcaggctcttttaacaagctgttggcttgtacgtaagtaggagggaaaagagtttcttttttcaagatttcatgagaaataaactaatgagactgaa NGG 10 8 17 8 24 0 100 5 10
    240827154159_0knz GTTCCCACATTCAGCAAGTTCATTCCTGAGGGCTCCCAGAGAGTGGGGCTGGTTGCCAGTCAGAAGAACGACCT(/C)GGACGCAGTGGCACTGATGCATCCCGATGGCTCTGCTGTTGTGGTCGTGCTAAACCGGTGAGGGCAATGGTGAGGT NGG 10 8 17 8 24 0 100 5 10
    240827085444_acmi cctggagaaggcttatcaaggcccagggaaatgaaagggagcctgttctggccctcggttccactcgctttctatttcacaggccttgctgtgctcctccttcatc(/g)cctctggtcagcggcttcatccctccttccttcagaggcgaggtaagtccgcttcgaaactgtgtctgcgcagctcctgcccgtgaggcgcggggctcag NGG 10 8 17 8 24 0 100 5 10