Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240913104224_lpj3 GGAGCGCCACGAGACGCTGGTCCAGTGGCGCGGCCGCCTCGGCGGGTCCGGGTTCGAGCCCGTGCACCTGGGATCCAACGCCTACAAGCAGGCAA(/T)GCACGCTGCTGGCCCTCTTCGCCGGCGGCGACGGGTACAGGGTGGAGGAGAAGGACGGGTGCCTGACTCTGGGATGGCATACGCGCCCGCTCATCGCCACCTCGGCGTGGCGCGTCGCCGCTCCGTGACGTGCCCACCGTCCGGCACAGCACAGCACAGCACAGCACAGTGGAAGCTAAGACGTCAGTGAACGCTGCTGAACTGCAG NGG 10 8 17 8 24 0 100 5 10
    240912154355_szbo CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240912154138_eb7m CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240912154116_ui7d GCAGTATGTGTGGTTGGGGAATTCATGTGGAGGTCAGAGTGGAAGCAGGTGAGAATGGAGGGGGCGGCAAAGGCTCGTTTCTGGGCATCTCTGCAGTCCTCCTCTGCTCCATGATGTGCACTTTGGGCGA(+ataa)GGAGAGTGCGTGCGTGTGAGAGGGTCCAGCAGAAGGAAACATGGCTGCCAAAGTGTTTGAGTCCATTGGCAAGTTTGGCCTGGCCTTAGCTGTTGCAGGAGGCGTGGTGAACTCTGCCTTATATAATGTGGATGCTGGGCACAGAGCTGTCATCTTTGACCGATTCCGTGGAGTGCAGGACATTGTGGTAGGGGAAGGGACTCATTTTCTCATCCCGTGG NGG 10 8 17 8 24 0 100 5 10
    240912153823_zohm CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240907060049_4cg4 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240905222626_dmap TCTACCCCTAGATATGCGGCCAAGTTTTAAATGTCCCTGGGGGAGGGGAAGGAGAAGGGACAGGGCTGGTGGAGCCCAGGCCTCTTGTCTCTGGGATCCCTCTCTCACGAGCCCTCCTGGTCTCTAGGCCCTCGGACCTCCGCGCTCCTG(+taaa)GCTTTCGCCCTGCTCTGCCTGCCCTGGACTCAGGAGGTGGGCGCCTTCCCAGCCATGCCCTTGTCCAGCCTATTTGCCAACGCCGTGCTCCGGGCCCAGCACCTGCACCAACTGGCTGCTGACACCTACAAGGAGT NGG 10 8 17 8 24 0 100 5 10
    240905222618_c9ho TCTACCCCTAGATATGCGGCCAAGTTTTAAATGTCCCTGGGGGAGGGGAAGGAGAAGGGACAGGGCTGGTGGAGCCCAGGCCTCTTGTCTCTGGGATCCCTCTCTCACGAGCCCTCCTGGTCTCTAGGCCCTCGGACCTCCGCGCTC(+taaa)CTGGCTTTCGCCCTGCTCTGCCTGCCCTGGACTCAGGAGGTGGGCGCCTTCCCAGCCATGCCCTTGTCCAGCCTATTTGCCAACGCCGTGCTCCGGGCCCAGCACCTGCACCAACTGGCTGCTGACACCTACAAGGAGT NGG 10 8 17 8 24 0 100 5 10
    240905222606_64rt TCTACCCCTAGATATGCGGCCAAGTTTTAAATGTCCCTGGGGGAGGGGAAGGAGAAGGGACAGGGCTGGTGGAGCCCAGGCCTCTTGTCTCTGGGATCCCTCTCTCACGAGCCCTCCTGGTCTCTAGGCCCTCGGACCTCCGCG(+taaa)CTCCTGGCTTTCGCCCTGCTCTGCCTGCCCTGGACTCAGGAGGTGGGCGCCTTCCCAGCCATGCCCTTGTCCAGCCTATTTGCCAACGCCGTGCTCCGGGCCCAGCACCTGCACCAACTGGCTGCTGACACCTACAAGGAGT NGG 10 8 17 8 24 0 100 5 10
    240905222554_y2mz TCTACCCCTAGATATGCGGCCAAGTTTTAAATGTCCCTGGGGGAGGGGAAGGAGAAGGGACAGGGCTGGTGGAGCCCAGGCCTCTTGTCTCTGGGATCCCTCTCTCACGAGCCCTCCTGGTCTCTAGGCCCTCGGACCTCC(+taaa)GCGCTCCTGGCTTTCGCCCTGCTCTGCCTGCCCTGGACTCAGGAGGTGGGCGCCTTCCCAGCCATGCCCTTGTCCAGCCTATTTGCCAACGCCGTGCTCCGGGCCCAGCACCTGCACCAACTGGCTGCTGACACCTACAAGGAGT NGG 10 8 17 8 24 0 100 5 10