Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241012174530_cih0 CGACCCGCGGCGGGGGCGTCTGCTGAT(/C)GGTCGCCTGCAACGCCGAGGACATGCTCCTCCCGCGATCCCACTTCACTTTCTACGGTCAGCTCGCCATCGCCTCGACCAACCACGCATTTTCCATTCGCTCCTCCAAAAAAAAAAATCACATTGAACGGCGTTTCCATGGCAGAGTTCGACG NGG 10 8 17 8 24 0 100 5 10
    241012174523_67g3 CGACCCGCGGCGGGGGCGTCTGCTGAT(/C)GGTCGCCTGCAACGCCGAGGACATGCTCCTCCCGCGATCCCACTTCACTTTCTACGGTCAGCTCGCCATCGCCTCGACCAACCACGCATTTTCCATTCGCTCCTCCAAAAAAAAAAATCACATTGAACGGCGTTTCCATGGCAGAGTTCGACG NGG 10 8 17 8 24 0 100 5 10
    241012174458_lufc TGCGTGACACTGTACCGGAGCAGCGGCCTCCACTTCGATCGATTCGGACCGAACTGATATGACGCTGGTGCGCGCGTGCGTGCGTGCGGTGCAGGGGTGTTCGACATGCCGGGCAAGAGGCTGCTGGCGCACTACAAGATCGACCCGCGGCGGGGGCGTCTGCTGAT(/C)GGTCGCCTGCAACGCCGAGGACATGCTCCTCCCGCGATCCCACTTCACTTTCTACGGTCAGCTCGCCATCGCCTCGACCAACCACGCATTTTCCATTCGCTCCTCCAAAAAAAAAAATCACATTGAACGGCGTTTCCATGGCAGAGTTCGACG NGG 10 8 17 8 24 0 100 5 10
    241012174323_c00e TGCGTGACACTGTACCGGAGCAGCGGCCTCCACTTCGATCGATTCGGACCGAACTGATATGACGCTGGTGCGCGCGTGCGTGCGTGCGGTGCAGGGGTGTTCGACATGCCGGGCAAGAGGCTGCTGGCGCACTACAAGATCGACCCGCGGCGGGGGCGTCTGCTGAT(/C)GGTCGCCTGCAACGCCGAGGACATGCTCCTCCCGCGATCCCACTTCACTTTCTACGGTCAGCTCGCCATCGCCTCGACCAACCACGCATTTTCCATTCGCTCCTCCAAAAAAAAAAATCACATTGAACGGCGTTTCCATGGCAGAGTTCGACG NGG 10 8 17 8 24 0 100 5 10
    241012174225_s01v CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    241011222015_ocbq ATGGATGTGACTTCCCAAGCCCGGGGCGTGGGCCTGGAGATGTACCCAGGCACCGCGCAGCCTGCGGCCCCCAACACCACCTCCCCCGAGCTCAACCTGTCCCACCCGCTCCTGGGCACCGCCCTGGCCA(/G)ATGGGACAGGTGAGCTCTCGGAGCACCAGCAGTACGTGATCGGCCTGTTCCTCTCGTGCCTCTACACCATCTTCCTCTTCCCCATCGGCTTTGTGGGCAACATCCTGATCCTGGTGGTGAACATCAGCTTCCGCGAGAAGATGACCATCCCCGACCTGTACTTCATCAACCTGGCGGTGGCGGACCTCATCCTGGTGGCCGACTCCCTCATTGAGGTGTTCAACCTGCACGAGCGGTACTACGACATCGCCGTCCTGTGCACCTTCATGTCGCTCTTCCTG NGG 10 8 17 8 24 0 100 5 10
    241011210921_klj9 CTACTGCTGAAAAGAGAGAGAGTGGACCTAAGCAAGCTGCAGTAAATGCTGCAGTTCAGAGGGTCCAGGTTCTTCCAGATGCTGATACTTTATTACATTT(-T)GCCACGGAAAGTACTCCAGATGGATTTTCTTGTTCATCCAGCCTGAGTGCTCTGAGCCTCGATGAGCCATTTATACAGAAAGATGTGGAATTAAGAATAA NG 10 8 17 8 24 0 100 5 10
    241011210915_p7ln CTACTGCTGAAAAGAGAGAGAGTGGACCTAAGCAAGCTGCAGTAAATGCTGCAGTTCAGAGGGTCCAGGTTCTTCCAGATGCTGATACTTTATTACATTT(-T)GCCACGGAAAGTACTCCAGATGGATTTTCTTGTTCATCCAGCCTGAGTGCTCTGAGCCTCGATGAGCCATTTATACAGAAAGATGTGGAATTAAGAATAA NG 10 8 17 8 24 0 100 5 10
    241011210853_occt CTACTGCTGAAAAGAGAGAGAGTGGACCTAAGCAAGCTGCAGTAAATGCTGCAGTTCAGAGGGTCCAGGTTCTTCCAGATGCTGATACTTTATTACATTT(-T)GCCACGGAAAGTACTCCAGATGGATTTTCTTGTTCATCCAGCCTGAGTGCTCTGAGCCTCGATGAGCCATTTATACAGAAAGATGTGGAATTAAGAATAA NGG 10 8 17 8 24 0 100 5 10
    241011202607_zkrp CTACTGCTGAAAAGAGAGAGAGTGGACCTAAGCAAGCTGCAGTAAATGCTGCAGTTCAGAGGGTCCAGGTTCTTCCAGATGCTGATACTTTATTACATTT(-T)GCCACGGAAAGTACTCCAGATGGATTTTCTTGTTCATCCAGCCTGAGTGCTCTGAGCCTCGATGAGCCATTTATACAGAAAGATGTGGAATTAAGAATAA NGG 10 8 17 8 24 0 100 5 10