Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241016213923_6bgn CAATGGAAACCTATCAGTGGACAACTGCTCCCCATATCTGGGTTCCTATTCTCCCCAGTCTCAGCCGATGGATCTGTATAGGTATCCAAGCCAAGACCCTCTGTCTAAGCTCAGTCTACCACCCATCCATACACTTTACCAGCCAAGGTTTGGAAATAGCCAGAGTTTTACATCTAAATACTTAGGTTATGGAAACCAAAATATGCAGGGAGATGGTTTCAGCAGTTGTACCATTAGACCAAATGTACATCATg(/C)taGGGAAATTGCCTCCTTATCCCACTCATGAGATGGATGGCCACTTCATGGGAGCCACCTCTAGATTACCACCCAATCTGAGCAATCCAAACATGGACTATAAAAATGGTGAACATCATTCACCTTCTCACATAATCCATAACTACAGTGCAGCTCCGGGCATGTTCAACAGCTCTCTTCATGCCCTGCATCTCCAAAACAAGGAGAATGACATGCTTTCCCACACAGCTAATGGGTTATCAAAGATGCTTCCAGCTCTTAACCATGATAGAACTGCTTGTGTCCAAGGAGGCTTACACAAATTAAGTGATGCTAATGGTCAGGAAAAGCAGCCATTGGCACTAGTCCAGGGTGTGGCTTCTGGTGCAGAGGACAACGATGAGGTCTGGTCAGACAGCGAGCAGAGCTTTCTGGATCCTGACATTGGGGGAGTGGCCGTGGCTCCAACTCATGGGTCAATTCTCattGAGTGTGCAAAGCGTGAGCTGCATGCCACAACCCCTTTAAAGAATCCCAATAGGAATCACCCCACCAGGATCTCCCTCGTCTTTTACCAGCATAAGAGCATGAATGAGCCAAAACATGGCTTGGCTCTTTGGGAAGCCAAAATGGCTGAAAAAGCCCGTGAGAAAGAGGAAGAGTGTGAAAAGTATGGCCCAGACTATGTGCCTCAGAAATCCCATGGCAAAAAAGTGAAACGGGAGCCTGCTGAGCCACATGAAACTTCAGAGCCCACTTACCTGCGTTTCATCAAGTCTCTTGCCGAAAGGACCATGTCCGTGACCACAGACTCCACAGTAACTACATCTCCATATGCCTTCACTCGGGTCACAGGGCCTTACAACAGATATATATGATATCACCCCCTTTTGTTGGTTACCTCACTTGAAAAGACCACAACCAACCTGTCAGTAGTATAGTTCTCATGACGTGGGCAGTGGGGAAAGGTCACAGTATTCATGACAAATGTGGTGGGAAAAACCTCAGCTCACCAGCAACAAAAGAGGTTATCTTACCATAGCACTTAATTTTCACTGGCTCCCAAGTGGTCACAGATGGCATCTAGGAAAAGACCAAAGCATTCTATGCAAAAAGAAGGTGGGGAAGAAAGTGTTCCGCAATTTACATTTTTAAACACTGGTTCTATTATTGGACGAGATGATATGTAAATGTGATCCCCCCCCCCCGCTTACAACTCTACACATCTGTGACCACTTTTAATAATATCAAGTTTGCATAGTCATGGAACACAAATCAAACAAGTACTGTAGTATTA NGG 10 8 17 8 24 0 100 5 10
    241016213254_cdg0 CCCAGCAGCAGCAGAGACCCCAGCAGCAGCAGCCACATCACCCTCAGACAGAGTCTGTCAACTCTTATTCTGCTTCTGGATCCACCAATCCATACATGAGACGGCCCAATCCAGTTAGTCCTTATCCAAACTCTTCACACACTTCAGATATCTATGGAAGCACCAGCCCTATGAACTTCTATTCCACCTCATCTCAAGCTGCAGGTTCATATTTGAATTCTTCTAATCCCATGAACCCTTACCCTGGGCTTTTGAATCAGAATACCCAATATCCATCATATCAATGCAATGGAAACCTATCAGTGGACAACTGCTCCCCATATCTGGGTTCCTATTCTCCCCAGTCTCAGCCGATGGATCTGTATAGGTATCCAAGCCAAGACCCTCTGTCTAAGCTCAGTCTACCACCCATCCATACACTTTACCAGCCAAGGTTTGGAAATAGCCAGAGTTTTACATCTAAATACTTAGGTTATGGAAACCAAAATATGCAGGGAGATGGTTTCAGCAGTTGTACCATTAGACCAAATGTACATCATg(/C)taGGGAAATTGCCTCCTTATCCCACTCATGAGATGGATGGCCACTTCATGGGAGCCACCTCTAGATTACCACCCAATCTGAGCAATCCAAACATGGACTATAAAAATGGTGAACATCATTCACCTTCTCACATAATCCATAACTACAGTGCAGCTCCGGGCATGTTCAACAGCTCTCTTCATGCCCTGCATCTCCAAAACAAGGAGAATGACATGCTTTCCCACACAGCTAATGGGTTATCAAAGATGCTTCCAGCTCTTAACCATGATAGAACTGCTTGTGTCCAAGGAGGCTTACACAAATTAAGTGATGCTAATGGTCAGGAAAAGCAGCCATTGGCACTAGTCCAGGGTGTGGCTTCTGGTGCAGAGGACAACGATGAGGTCTGGTCAGACAGCGAGCAGAGCTTTCTGGATCCTGACATTGGGGGAGTGGCCGTGGCTCCAACTCATGGGTCAATTCTCATTGAGTGTGCAAAGCGTGAGCTGCATGCCACAACCCCTTTAAAGAATCCCAATAGGAATCACCCCACCAGGATCTC NGG 10 8 17 8 24 0 100 5 10
    241016212947_avwd ataaaaattaactccaaataaatccatttatatgaaattctagaaaatgaaaatctgtagtgatagattagtagttgtctgagaacaaagcaggaagcatgaattatacaggggcatgaggaaatttttaagagtaatgaatatgtactttattttggttgtgacaaatatatatcaaaactcaaatagcatactttatggcctcaataacactataaaataaaaattttaccatgtcaagatatttgctctattttgtgtcattccattttgtttctggatatatatttaagttcaaaacatttttttaaagttctaaatggtctaaatactagtgagttttcggtgtaagagtaaaactaactactttcgcattcacacacacttttatttttcagATTGAATATGAACACAGAGCACCAGAGTGCCGTCTGGGTCTGAAGGAAGGCCG(/A)TCCATTCTCAGGGGTCACTGCATGTTTGGACTTCTGTGCTCATGCCCACAGAGACTTGCACAACATGCAGAATGGCAGCACATTGgtaagttgggctgaggacagcttagcagctgttgagtctgttctcacactgctaataaagacatatgcaagactgggtaatttataaaggaaagagatttaattgactcacagttccacatggctgtggaggcctcacaatcatagctgaaggcaaatgaggagcaaagtcacatcttacatggcggcaggcaagagaacatgtgcaggggaactcccctttataaaatcatcagatctcatgagacttactctcctgagaacagcatgggaaagatctgcccccatgattcaattacctcccactgggtccttcccaaaacacatgggaattttgggagctacaattcaagatgagatttaggt NGG 10 8 17 8 24 0 100 5 10
    241016212757_0t14 GGAAGTACAGAATATAAATCGTAGAAATTCCCCTTATAGTCAGACCATGAAATCAAGTGCATGCAAAATACAGGTTTCTTGTTCAAACAATACACACCTAGTTTCAGAGAATAAAGAACAGACTACACATCCTGAACTTTTTGCAGGAAACAAGACCCAAAACTTGCATCACATGCAATATTTTCCAAATAATGTGATCCCAAAGCAAGATCTTCTTCACAGGTGCTTTCAAGAACAGGAGCAGAAGTCACAACAAGCTTCAGTTCTACAGGGATATAAAAATAGAAACCAAGATATGTCTGGTCAACAAGCTGCGCAACTTGCTCAGCAAAGGTACTTGATACATAACCATGCAAATGTTTTTCCTGTGc(/T)ctGACCAGGGAGGAAGTCACACTCAGACCCCTCCCCAGAAGGACACTCAAAAGCATGCTGCTCTAAGGTGGCATCTCTTACAGAAGCAAGAACAGCAGCAAACACAGCAACCCCAAACTGAGTCTTGCCATAGTCAGATGCACAGGCCAATTAAGGTGGAACCTGGATGCAAGCCACATGCCTGTATGCACACAGCACCACCAGAAAACAAAACATGGAAAAAGGTAACTAAGCAAGAGAATCCACCTGCAAGCTGTGATAATGTGCAGCAAAAGAGCATCATTGAGACCATGGAGCAGCATCTGAAGCAGTTTCACGCCAAGTCGTTATTTGACCATAAGGCTCTTACTCTCAAATCACAGAAGCAAGTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAACCACTGCTGCAGAACTTGATAGCCACACCCCAGCTTTAGAGCAGCAAACAACTTCTTCAGAAAAGACACCAACCAAAAGAACAGCTGCTTCTGTTCTCAATAATTTTATAGAGTCACCTTCCA NGG 10 8 17 8 24 0 100 5 10
    241016212420_t4df ggaaatccttcagcttctgaagtcaggcaggagttcggctcctttttcgccttccctgctcttgatggggaggatgctagggctacgtgttcgttacatgcagactccaggtgtacgttgccgagctaaccctgcaggagaggagaactagagagtgtgtcctggaggtatttctgagggattgttcaaaattactcttatttctgctgggttgtgaaactctaggcagtgatgaccttactacctttaaggtcacagaaaccagcacagtgcctggcacatggttggtgatctgagtgccgggttgtttataaaggacagaagattcggcagaactaagcaggcgtcagaggagttggtgggtgtgagtgcccctgtccctgcacttcgggtggctgctggtcctccgggtcctgctgtgtggttagacggcttccgggcagcctggtctggccagcactcaccctgccctctctgccttttctcccccagGGTATTTGGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGCCGGTCATG(/C)GAGCGTGCCAGTCATCCGCCACCTCTTCGCTCCGCTGAAGGAGTATTTTGCGTGTGTGTAAGGGACATGGGGGCAAACTGAGGTAGCGACACAAAGTTAAACAAACAAACAAAAAACACAAAACATAATAAAACACCAAGAACATGAGGATGGAGAGAAGTATCAGCACCCAGAAGAGAAAAAGGAATTTAAAACAAAAACCACAGAGGCGGAAATACCGGAGGGCTTTGCCTTGCGAAAAGGGTTGGACATCATCTCCTGATTTTTCAATGTTATTCTTCAGTCCTATTTAAAAACAAAACCAAGCTCCCTTCCCTTCCTCCCCCTTCCCTTTTTTTTCGGTCAGACCTTTTATTTTCTACTCTTTTCAGAGGGGTTTTCTGTTTGTTTGGGTTTTGTTTCTTGCTGTGACTGAAA NGG 10 8 17 8 24 0 100 5 10
    241016212258_y4vo ggaaatccttcagcttctgaagtcaggcaggagttcggctcctttttcgccttccctgctcttgatggggaggatgctagggctacgtgttcgttacatgcagactccaggtgtacgttgccgagctaaccctgcaggagaggagaactagagagtgtgtcctggaggtatttctgagggattgttcaaaattactcttatttctgctgggttgtgaaactctaggcagtgatgaccttactacctttaaggtcacagaaaccagcacagtgcctggcacatggttggtgatctgagtgccgggttgtttataaaggacagaagattcggcagaactaagcaggcgtcagaggagttggtgggtgtgagtgcccctgtccctgcacttcgggtggctgctggtcctccgggtcctgctgtgtggttagacggcttccgggcagcctggtctggccagcactcaccctgccctctctgccttttctcccccagGGTATTTGGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCG(/A)CTTGGCGAGGCAGAGACTGCTGGGCCGGTCATGGAGCGTGCCAGTCATCCGCCACCTCTTCGCTCCGCTGAAGGAGTATTTTGCGTGTGTGTAAGGGACATGGGGGCAAACTGAGGTAGCGACACAAAGTTAAACAAACAAACAAAAAACACAAAACATAATAAAACACCAAGAACATGAGGATGGAGAGAAGTATCAGCACCCAGAAGAGAAAAAGGAATTTAAAACAAAAACCACAGAGGCGGAAATACCGGAGGGCTTTGCCTTGCGAAAAGGGTTGGACATCATCTCCTGATTTTTCAATGTTATTCTTCAGTCCTATTTAAAAACAAAACCAAGCTCCCTTCCCTTCCTCCCCCTTCCCTTTTTTTTCGGTCAGACCTTTTATTTTCTACTCTTTTCAGAGGGGTTTTCTGTTTGTTTGGGTTTTGTTTCTTGCTGTGACTGAAA NGG 10 8 17 8 24 0 100 5 10
    241016212133_siti catggttgcagctagggtcgtgagcctgcagagcagctgggtctcctctctttcgtgtcaaaggacttctttgccaagttcacagataatttctctttcttcctgtctgcctctgtccctggacagcaggcccatcacgttgcctttatcctcccagATCCAGGAGTGGGGCCCATTCGATCTGGTGATTGGGGGCAGTCCCTGCAATGACCTCTCCATCGTCAACCCTGCTCGCAAGGGCCTCTACGgtaggtaccatcctgtcccctccaccttctcagctggtgcttccgcacataggcttcctccttggatatttctgccctgggacagctattcccgatgaccctgtcttcccgtgccctccccgtgcccgagccacaccactgtcctatgcagacagccccagctgatggctttctcttccgacctctcagAGGGCACTGGCCG(/A)GCTCTTCTTTGAGTTCTACCGCCTCCTGCATGATGCGCGGCCCAAGGAGGGAGATGATCGCCCCTTCTTCTGGCTCTTTGAGAATGTGGTGGCCATGGGCGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGgtatagccagcaaccttggtttggccagctcactaatggcttctaccttggactgctgcttcatcctgtctcatctgcattgtggagctggggactggtgacttctgctttgcaaggggcctgtttgggaagccccaggcttcctggatagaaagggaggaagcagggttagaggcctacctcggaaagaaccagtggtctcactctaagaggtggcattgtgggtgtggacctccggggttgaaggacctgtcactcaatcaggtggggtgccagactcctggggttgggtattctgtatggtgtttgttacac NGG 10 8 17 8 24 0 100 5 10
    241016212003_za5s AGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGGGGGCTGCCCAGGCAGCCATTAAGGAAGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCCAGATGTTCTTCGCTAATAACCACGACCAGGAATTTGtgagtgctgggcctggggcgcggtctcgagctcccttggctgagccttggttgtggggcctgagctgtgcgcagggtgtgtgggtctaggagcctggctggagggccagcgctgggtgggagcttgggacaccgctgggcctgcatctgacctgttgtgctcactgcttaggACCCTCCAAAGGTTTACCCACCTGTCCCAGCTGAGAAGAGGAAGCCCATCC(/T)GGGTGCTGTCTCTCTTTGATGGAATCGCTAcaggtgaggggtgcaggcccaagaggtgctggccgccttgtcccaggatgggggagccaggtgtgtgggcaactctggcaaaacgaaatgatggttagcttcacagctgttaaaatattatcccaaaggggaagcttgtgtgtgaagtggaaacgtatgcagtccgtggacttagatttgaccaaaaataaaaaagtaaaacaggtggtaaagcagcctgcctgctgggcgcttccttagtaaggaagattcagccacagaggccgagtgatcattagtcagattcacagagatttcacagagcctcaaaggacagattcagcaagacagaaacatacacagttctgttgtgttttgattatacacaagatagtctaaagaagacttatttaaatcacccattatccttttattaatattttggcatatgtccttttcagatttttttctgtgcgtttgtgtttatataaccatacatccatagatagataaattaagtgggtagtgaatgcacacggttttgtaacttgccttgcatttattatattatga NGG 10 8 17 8 24 0 100 5 10
    241016211739_jfsf GCTGAGAAGAAAGCCAAGGTCATTGCAGGAATGAATGCTGTGGAAGAAAACCAGGGGCCCGGGGAGTCTCAGAAGGTGGAGGAGGCCAGCCCTCCTGCTGTGCAGCAGCCCACTGACCCCGCATCCCCCACTGTGGCTACCACGCCTGAGCCCGTGGGGTCCGATGCTGGGGACAAGAATGCCACCAAAGCAGGCGATGACGAGCCAGAGTACGAGgtgagcggcttcttcaccagccgtggccagctctttccgggggccttccactgccagctcctcccgtcctgctctcctctctccatctcccacgggccgctcgctctctccacctgaccccgggcctcttcgtctccccgattttgctctgtcttgcctcattcagatggagctccctcctggctgtcctgccccccagatgcccagggctggggttgccatggggggatcagggtggcagggcctcgtgaccactgtgtaatgatttctgctccttggggctccagGACGGCCGGGGCTTTGGCATTGGGGAGCTGGTGTGGGGGAAACTGCGGGGCTTCTCCTGGTGGCCAGGCCGCATTGTGTCTTGGTGGATGACGGGCCGGAGCCGAGCAGCTGAAGGCACCCG(/A)CTGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGgtaagttgtggggtttggcagtagcctggggtgggggaaggttcttgatcccagggcctgtggttttggctgggggcagtctggaattggggtgatggaagtgggttctgctcacccctactctgcctgtggtgggtggaagaagctgtagggataattaggttgcacctgtggctcagagagctaggatgctggcccagttttagccttccaggaacctattcactagaattcctttccacctgcactcaggtacttccctactcttggtctggactcctctttgcatcgggtaattatttatcactgtatctggtcccctccagggctgagactgactctcgaggctcccagcccctgccagttgcaaggcatggggtgggtgcttgcaagtgtaagcctcggcaaacaaggcctggcttgtccccccag NGG 10 8 17 8 24 0 100 5 10
    241016210905_41ba atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacAC(/T)aTggTgtTcaAtgTttTTCAcgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa NGG 10 8 17 8 24 0 100 5 10