Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241025013601_t0up tttctctcaccctctctcttcagggtgacca(/g)gggctttccagggcccctgggtgaggctggagagaaggtaagtgcaacct NGG 10 8 17 8 24 0 100 5 10
    241023095429_5diq TGCTCCCAGCAAATGTGTGTTTGCTGCTCTGTTTCCCTGCGTGTTTCTTGCCTGTCTTGTGTGTATCTTTGTGTCTGGGGCCATCCTGCCCTGTGCTGCCCAACCAAGCCATCTCTGCCCACAGGTGCCCGCCTGGCTGGGTGGGTGAGCG(/C)GTGTCAGCTGGAGGACCCCTGTCACTCAGGCCCCTGTGCTGGCCGTGGTGTCTGCCAGAGTTCAGTGGTGGCTGGCACCGCCCGATTCTCATGCCGGTGCCCCCGTGGCTTCCGAGGTGAGAGGGGAAGAGTCTGGAGGGGAGGTAGTCGGGGGTGTGGTCAGTCCTAAACTCACCCTGTCCTGGTCCCTCCAGGCCCTGACTGCTCCCTGCCAGATCCCTGCCTC NGG 10 8 17 8 24 0 100 5 10
    241021190404_bru8 cggtgcctaccatggctgatgacctgggggagccagccccactctggccacatgaccctgacgccaaggagaagcccctccacatgcctgggggagatggtcaccgggggaaaagcctgaa(/c)gctgctggagaagatccctgaagatgctgaggccacggttgtgcttgtgggttgtgtgcctttcttggagcagcctgcagcagccttcgtgcgtctgaatgaggctgtactcctggagt NGG 10 8 17 8 24 0 100 5 10
    241021185645_mnvk cggtgcctaccatggctgatgacctgggggagccagccccactctggccacatgaccctgacgccaaggagaagcccctccacatgcctgggggagatggtcaccgggggaaaagcctgaa(/c)gctgctggagaagatccctgaagatgctgaggccacggttgtgcttgtgggttgtgtgcctttcttggagcagcctgcagcagccttcgtgcgtctgaatgaggctgtactcctggagt NGG 10 8 17 8 24 0 100 5 10
    241021142332_cjoz CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    241021142323_nk6b CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    241021124838_ff5u CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    241021113725_cxic gttaagaatatgtacccggaaccagagataggacctactttccggccaaaccaatgatattatgtttgtcttctccagtgccaaccatccagaggacaagatggattggggcacactccagagcatcctcgggg(+G)tgtcaacaaacactccaccagcattggaaagatctggctcacggtcctcttcatcttccgcatcatgatcctcgtggtggctgcaaaggaggtgtggggagatgagcaagccgattttgtctgcaacacgctccagcctggctgcaagaatgtatgctacgaccacc NGG 10 8 17 8 24 0 100 5 10
    241017203944_x7ap CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    241016214202_8qrz tgtataatccttcttagaatgtgaagttgtccttaaacttttaaataccttcagttaatctttatattgtcatttatgaaaaccttgaactaagacttatgtatctttcatctagctctggttttaatgcaggtagcatttaattgtccccactgtactgggtatagtctgctaaacattaaggagtagttttgcatctctccttgttctgatactagggtcaaagcccactttttatagatgggcagcaaaaggcacattggacatgctgataaatgttgccctaattgtgatctaaacatgataaaatatacatacataagtgcccttatctgctgcaagtgacccttgttttgttttggttggggtggggggtgtttgggatggaatggtgatccacgcagGTGGTTCGCAGAAGCAGCAGTGAAGAGAAGCTACTGtgtttggtgC(/T)GGgagcgagctggCCACACCTGTGAGGCTGCAGTGATTGTGATTCTCATCCTGGTGTGGGAAGGAATCCCGCTGTCTCTGGCTGACAAACTCTACTCGGAGCTTACCGAGACGCTGAGGAAATACGGCACGCTCACCAATCGCCGGTGTGCCTTGAATGAAGAgtaagtgaagcccagggcctctcccctctttgcggccactgataggaaagcccaatctttggttgaaaggaagagagttcagcgtgcacttttacatttataaaatgggcatcaaaatgcctgtttggcagtcatgcgataagaagttgtatttgctaatgtgaataacttgagatgatttcattatctgaattgtacagtttagccattaattaggagcagtcagagtgtctgtaaccacatggcctcagttataccataaacttgaaattgtttatgtgctcacatgctacaagtgacggctcctgtgtgcctggccactatattagtatgtattgactccacttccatgttgcagtatctgaaacagaaagtaag NGG 10 8 17 8 24 0 100 5 10