Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241030170253_zx38 AGAACTGCATAAACAAGGTATTTATCCATGCAGCTATTGCAGAAGGCAGCTAGGGGAAGCCCCTACCTCTGCTTGTGCTTGCAGGGCCTGCAGTCTGAGAGCTGGAGCCATCTTCCAGCTCAGCCAGTGCCCCCCCCCCCCCCATGTGCCC(+TTATGTAACAT)TGGTGACACTGGGCCCTGCAGCAGAGCGGGGGGTTCACAGGTGAACCCT NGG 10 8 17 8 24 0 100 5 10
    241030170052_k4je AGAACTGCATAAACAAGGTATTTATCCATGCAGCTATTGCAGAAGGCAGCTAGGGGAAGCCCCTACCTCTGCTTGTGCTTGCAGGGCCTGCAGTCTGAGAGC(+TTATGTAACAT)TGGAGCCATCTTCCAGCTCAGCCAGTGCCCCCCCCCCCCCCATGTGCCCTGGTGACACTGGGCCCTGCAGCAGAGCGGGGGGTTCACAGGTGAACCCT NGG 10 8 17 8 24 0 100 5 10
    241030170039_zzon AGAACTGCATAAACAAGGTATTTATCCATGCAGCTATTGCAGAAGGCAGCTAGGGGAAGCCCCTACCTCTGCTTGTGCTTGCA(+TTATGTAACAT)GGGCCTGCAGTCTGAGAGCTGGAGCCATCTTCCAGCTCAGCCAGTGCCCCCCCCCCCCCCATGTGCCCTGGTGACACTGGGCCCTGCAGCAGAGCGGGGGGTTCACAGGTGAACCCT NGG 10 8 17 8 24 0 100 5 10
    241030170029_zxkb AGAACTGCATAAACAAGGTATTTATCCATGCAGCTATTGCAGAAGGCAGCTAGGGGAAGCCCCTACCTCTGCTTGTGCTTGC(+TTATGTAACAT)AGGGCCTGCAGTCTGAGAGCTGGAGCCATCTTCCAGCTCAGCCAGTGCCCCCCCCCCCCCCATGTGCCCTGGTGACACTGGGCCCTGCAGCAGAGCGGGGGGTTCACAGGTGAACCCT NGG 10 8 17 8 24 0 100 5 10
    241030165940_0ko2 AGAACTGCATAAACAAGGTATTTATCCATGCAGCTATTGCAGAAGGCAGCTAGG(+TTATGTAACAT)GGAAGCCCCTACCTCTGCTTGTGCTTGCAGGGCCTGCAGTCTGAGAGCTGGAGCCATCTTCCAGCTCAGCCAGTGCCCCCCCCCCCCCCATGTGCCCTGGTGACACTGGGCCCTGCAGCAGAGCGGGGGGTTCACAGGTGAACCCT NGG 10 8 17 8 24 0 100 5 10
    241030165850_qskn AGAACTGCATAAACAAGGTATTTATCCATGCAGCTATTGCAGAAGGCAGCTAG(+TTATGTAACAT)GGGAAGCCCCTACCTCTGCTTGTGCTTGCAGGGCCTGCAGTCTGAGAGCTGGAGCCATCTTCCAGCTCAGCCAGTGCCCCCCCCCCCCCCATGTGCCCTGGTGACACTGGGCCCTGCAGCAGAGCGGGGGGTTCACAGGTGAACCCT NGG 10 8 17 8 24 0 100 5 10
    241030165808_hryr AGAACTGCATAAACAAGGTATTTATCCATGCAGCTATTGCAGAAGGCAGCTA(+TTATGTAACAT)GGGGAAGCCCCTACCTCTGCTTGTGCTTGCAGGGCCTGCAGTCTGAGAGCTGGAGCCATCTTCCAGCTCAGCCAGTGCCCCCCCCCCCCCCATGTGCCCTGGTGACACTGGGCCCTGCAGCAGAGCGGGGGGTTCACAGGTGAACCCT NGG 10 8 17 8 24 0 100 5 10
    241030165756_mqmk AGAACTGCATAAACAAGGTATTTATCCATGCAGCTATTGCAGAAGGCAGCT(+TTATGTAACAT)AGGGGAAGCCCCTACCTCTGCTTGTGCTTGCAGGGCCTGCAGTCTGAGAGCTGGAGCCATCTTCCAGCTCAGCCAGTGCCCCCCCCCCCCCCATGTGCCCTGGTGACACTGGGCCCTGCAGCAGAGCGGGGGGTTCACAGGTGAACCCT NGG 10 8 17 8 24 0 100 5 10
    241030165702_jfd5 AGAACTGCATAAACAAGGTATTTATCCATGCAGCTATTGCAGAAGGCAGC(+TTATGTAACAT)TAGGGGAAGCCCCTACCTCTGCTTGTGCTTGCAGGGCCTGCAGTCTGAGAGCTGGAGCCATCTTCCAGCTCAGCCAGTGCCCCCCCCCCCCCCATGTGCCCTGGTGACACTGGGCCCTGCAGCAGAGCGGGGGGTTCACAGGTGAACCCT NGG 10 8 17 8 24 0 100 5 10
    241030165649_tsmj AGAACTGCATAAACAAGGTATTTATCCATGCAGCTATTGCAGAAGGCAG(+TTATGTAACAT)CTAGGGGAAGCCCCTACCTCTGCTTGTGCTTGCAGGGCCTGCAGTCTGAGAGCTGGAGCCATCTTCCAGCTCAGCCAGTGCCCCCCCCCCCCCCATGTGCCCTGGTGACACTGGGCCCTGCAGCAGAGCGGGGGGTTCACAGGTGAACCCT NGG 10 8 17 8 24 0 100 5 10