Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241030164705_r9o3 ATACTGTGGAGGGCTGGCCGGGCAGTCGGGTGCAAGGAAGAAGCCAGAAACCA(+TTATGTAACAT)GAAGTCTTTTAATATTCCCCCGATGCCACCCGAAAGAGCATTTGAGC NGG 10 8 17 8 24 0 100 5 10
    241030164653_wb8h ATACTGTGGAGGGCTGGCCGGGCAGTCGGGTGCAAGGAAGAAGCCA(+TTATGTAACAT)GAAACCAGAAGTCTTTTAATATTCCCCCGATGCCACCCGAAAGAGCATTTGAGC NGG 10 8 17 8 24 0 100 5 10
    241030164409_ebfd ATACTGTGGAGGGCTG(+TTATGTAACAT)GCCGGGCAGTCGGGTGCAAGGAAGAAGCCAGAAACCAGAAGTCTTTTAATATTCCCCCGATGCCACCCGAAAGAGCATTTGAGC NGG 10 8 17 8 24 0 100 5 10
    241030164316_fr35 ATACTGTGGAGGGCTGGCCGGGCAGTCGGGTGCA(+TTATGTAACAT)AGGAAGAAGCCAGAAACCAGAAGTCTTTTAATATTCCCCCGATGCCACCCGAAAGAGCATTTGAGC NGG 10 8 17 8 24 0 100 5 10
    241030164303_168j ATACTGTGGAGGGCTGGCCGGGCAGTC(+TTATGTAACAT)GGGTGCAAGGAAGAAGCCAGAAACCAGAAGTCTTTTAATATTCCCCCGATGCCACCCGAAAGAGCATTTGAGC NGG 10 8 17 8 24 0 100 5 10
    241030164235_nwo7 ATACTGTGGAGGGCTGGCCGGGCAGT(+TTATGTAACAT)CGGGTGCAAGGAAGAAGCCAGAAACCAGAAGTCTTTTAATATTCCCCCGATGCCACCCGAAAGAGCATTTGAGC NGG 10 8 17 8 24 0 100 5 10
    241030164223_edcw ATACTGTGGAGGGCTGGCC(+TTATGTAACAT)GGGCAGTCGGGTGCAAGGAAGAAGCCAGAAACCAGAAGTCTTTTAATATTCCCCCGATGCCACCCGAAAGAGCATTTGAGC NGG 10 8 17 8 24 0 100 5 10
    241030164200_hj6k ATACTGTGGAGGGCTGGC(+TTATGTAACAT)CGGGCAGTCGGGTGCAAGGAAGAAGCCAGAAACCAGAAGTCTTTTAATATTCCCCCGATGCCACCCGAAAGAGCATTTGAGC NGG 10 8 17 8 24 0 100 5 10
    241030163709_g8k9 ATACTGTGGAGGGCTGGC(+TTATGTAACAT)CGGGCAGTCGGGTGCAAGGAAGAAGCCAGAAACCAGAAGTCTTTTAATATTCCCCCGATGCCACCCGAAAGAGCATTTGAGC NGG 10 8 17 8 24 0 100 5 10
    241030163457_qb4j ATACTGTGGAGGGCTGGCCGGGCAGTCGGGTGCAAGGAAGAAGCCAGAAACCAGAAGTCTTTTAATATT(+TTATGTAACAT)CCCCCGATGCCACCCGAAAGAGCATTTGAGC NGG 10 8 17 8 24 0 100 5 10